Human cartilage fragments in a composite scaffold for single-stage cartilage repair: An in vitro study of the chondrocyte migration and the influence of TGF-β1 and G-CSF

A. Marmotti, D. E. Bonasia, M. Bruzzone, R. Rossi, F. Castoldi, G. Collo, C. Realmuto, C. Tarella, G. M. Peretti

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Purpose: Minced chondral fragments are becoming popular as a source of cells for cartilage repair, as a growing interest is developing towards one-stage procedures to treat cartilage lesions. The purpose of this study is to (A) compare cell outgrowth from cartilage fragments of adult and young donors using two different types of scaffolds and (B) evaluate the influence of transforming-growth-factor-β1 (TGF-β1) and granulocyte colony-stimulating factor (G-CSF) on chondrocyte behaviour. Methods: In part (A) cartilage fragments from adult and young donors were either loaded onto an HA-derivative injectable paste scaffold or onto an HA-derivative membrane scaffold. Construct sections were then examined for cell counting after 1, 2 and 3 months. In part (B) only membrane scaffolds were prepared using cartilage fragments from young donors. Constructs were cultured either in standard growth medium or in the presence of specific growth factors, such as TGF-β1 or G-CSF or TGF-β1 + G-CSF. After 1 month, construct sections were examined for cell counting. Expression of chondrocyte markers (SOX9, CD151, CD49c) and proliferative markers (β-catenin, PCNA) was assessed using immunofluorescence techniques, both in unstimulated construct sections and in cells from unstimulated and stimulated construct cultures. Results: Part (A): histological analysis showed age-dependent and time-dependent chondrocyte migration. A significant difference (p <0.05) was observed between young and older donors at the same time point. No difference was detected between the two types of scaffolds within the same group at the same time point. Part (B): after 1 month, the number of migrating cells/area significantly increased due to exposure to TGF-β1 and/or G-CSF (p <0.05). Immunofluorescence revealed that outgrowing cells from unstimulated scaffold sections were positive for SOX9, CD151, CD49c and G-CSF receptor. Immunofluorescence of cells from construct cultures showed an increase in β-catenin in all stimulated groups and an increased PCNA expression in G-CSF-exposed cultures (p <0.05). Conclusion: Outgrowing cells may represent a subset of chondrocytes undergoing a phenotypic shift towards a proliferative state. TGF-β1, and to a greater extent G-CSF, may accelerate this outgrowth. The clinical relevance of this study may involve a potential future clinical application of scaffolds preloaded with growth factors as an additional coating for chondral fragments. Indeed, a controlled delivery of G-CSF, widely employed in various clinical settings, might improve the repair process driven by minced human cartilage fragments during one-stage cartilage repair.

Original languageEnglish
Pages (from-to)1819-1833
Number of pages15
JournalKnee Surgery, Sports Traumatology, Arthroscopy
Volume21
Issue number8
DOIs
Publication statusPublished - Aug 2013

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Transforming Growth Factors
Granulocyte Colony-Stimulating Factor
Chondrocytes
Cartilage
Tissue Donors
Fluorescent Antibody Technique
Catenins
Proliferating Cell Nuclear Antigen
Young Adult
Intercellular Signaling Peptides and Proteins
Granulocyte Colony-Stimulating Factor Receptors
In Vitro Techniques
Membranes
Ointments
Cell Culture Techniques
Cell Count
Injections
Growth

Keywords

  • Cartilage repair
  • Fibrin glue
  • G-CSF
  • G-CSF receptor
  • Hyaluronic acid
  • In vitro human
  • Minced cartilage fragments
  • Platelet-rich plasma
  • Scaffold
  • TGF-β1

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Human cartilage fragments in a composite scaffold for single-stage cartilage repair : An in vitro study of the chondrocyte migration and the influence of TGF-β1 and G-CSF. / Marmotti, A.; Bonasia, D. E.; Bruzzone, M.; Rossi, R.; Castoldi, F.; Collo, G.; Realmuto, C.; Tarella, C.; Peretti, G. M.

In: Knee Surgery, Sports Traumatology, Arthroscopy, Vol. 21, No. 8, 08.2013, p. 1819-1833.

Research output: Contribution to journalArticle

Marmotti, A. ; Bonasia, D. E. ; Bruzzone, M. ; Rossi, R. ; Castoldi, F. ; Collo, G. ; Realmuto, C. ; Tarella, C. ; Peretti, G. M. / Human cartilage fragments in a composite scaffold for single-stage cartilage repair : An in vitro study of the chondrocyte migration and the influence of TGF-β1 and G-CSF. In: Knee Surgery, Sports Traumatology, Arthroscopy. 2013 ; Vol. 21, No. 8. pp. 1819-1833.
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T2 - An in vitro study of the chondrocyte migration and the influence of TGF-β1 and G-CSF

AU - Marmotti, A.

AU - Bonasia, D. E.

AU - Bruzzone, M.

AU - Rossi, R.

AU - Castoldi, F.

AU - Collo, G.

AU - Realmuto, C.

AU - Tarella, C.

AU - Peretti, G. M.

PY - 2013/8

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N2 - Purpose: Minced chondral fragments are becoming popular as a source of cells for cartilage repair, as a growing interest is developing towards one-stage procedures to treat cartilage lesions. The purpose of this study is to (A) compare cell outgrowth from cartilage fragments of adult and young donors using two different types of scaffolds and (B) evaluate the influence of transforming-growth-factor-β1 (TGF-β1) and granulocyte colony-stimulating factor (G-CSF) on chondrocyte behaviour. Methods: In part (A) cartilage fragments from adult and young donors were either loaded onto an HA-derivative injectable paste scaffold or onto an HA-derivative membrane scaffold. Construct sections were then examined for cell counting after 1, 2 and 3 months. In part (B) only membrane scaffolds were prepared using cartilage fragments from young donors. Constructs were cultured either in standard growth medium or in the presence of specific growth factors, such as TGF-β1 or G-CSF or TGF-β1 + G-CSF. After 1 month, construct sections were examined for cell counting. Expression of chondrocyte markers (SOX9, CD151, CD49c) and proliferative markers (β-catenin, PCNA) was assessed using immunofluorescence techniques, both in unstimulated construct sections and in cells from unstimulated and stimulated construct cultures. Results: Part (A): histological analysis showed age-dependent and time-dependent chondrocyte migration. A significant difference (p <0.05) was observed between young and older donors at the same time point. No difference was detected between the two types of scaffolds within the same group at the same time point. Part (B): after 1 month, the number of migrating cells/area significantly increased due to exposure to TGF-β1 and/or G-CSF (p <0.05). Immunofluorescence revealed that outgrowing cells from unstimulated scaffold sections were positive for SOX9, CD151, CD49c and G-CSF receptor. Immunofluorescence of cells from construct cultures showed an increase in β-catenin in all stimulated groups and an increased PCNA expression in G-CSF-exposed cultures (p <0.05). Conclusion: Outgrowing cells may represent a subset of chondrocytes undergoing a phenotypic shift towards a proliferative state. TGF-β1, and to a greater extent G-CSF, may accelerate this outgrowth. The clinical relevance of this study may involve a potential future clinical application of scaffolds preloaded with growth factors as an additional coating for chondral fragments. Indeed, a controlled delivery of G-CSF, widely employed in various clinical settings, might improve the repair process driven by minced human cartilage fragments during one-stage cartilage repair.

AB - Purpose: Minced chondral fragments are becoming popular as a source of cells for cartilage repair, as a growing interest is developing towards one-stage procedures to treat cartilage lesions. The purpose of this study is to (A) compare cell outgrowth from cartilage fragments of adult and young donors using two different types of scaffolds and (B) evaluate the influence of transforming-growth-factor-β1 (TGF-β1) and granulocyte colony-stimulating factor (G-CSF) on chondrocyte behaviour. Methods: In part (A) cartilage fragments from adult and young donors were either loaded onto an HA-derivative injectable paste scaffold or onto an HA-derivative membrane scaffold. Construct sections were then examined for cell counting after 1, 2 and 3 months. In part (B) only membrane scaffolds were prepared using cartilage fragments from young donors. Constructs were cultured either in standard growth medium or in the presence of specific growth factors, such as TGF-β1 or G-CSF or TGF-β1 + G-CSF. After 1 month, construct sections were examined for cell counting. Expression of chondrocyte markers (SOX9, CD151, CD49c) and proliferative markers (β-catenin, PCNA) was assessed using immunofluorescence techniques, both in unstimulated construct sections and in cells from unstimulated and stimulated construct cultures. Results: Part (A): histological analysis showed age-dependent and time-dependent chondrocyte migration. A significant difference (p <0.05) was observed between young and older donors at the same time point. No difference was detected between the two types of scaffolds within the same group at the same time point. Part (B): after 1 month, the number of migrating cells/area significantly increased due to exposure to TGF-β1 and/or G-CSF (p <0.05). Immunofluorescence revealed that outgrowing cells from unstimulated scaffold sections were positive for SOX9, CD151, CD49c and G-CSF receptor. Immunofluorescence of cells from construct cultures showed an increase in β-catenin in all stimulated groups and an increased PCNA expression in G-CSF-exposed cultures (p <0.05). Conclusion: Outgrowing cells may represent a subset of chondrocytes undergoing a phenotypic shift towards a proliferative state. TGF-β1, and to a greater extent G-CSF, may accelerate this outgrowth. The clinical relevance of this study may involve a potential future clinical application of scaffolds preloaded with growth factors as an additional coating for chondral fragments. Indeed, a controlled delivery of G-CSF, widely employed in various clinical settings, might improve the repair process driven by minced human cartilage fragments during one-stage cartilage repair.

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KW - Fibrin glue

KW - G-CSF

KW - G-CSF receptor

KW - Hyaluronic acid

KW - In vitro human

KW - Minced cartilage fragments

KW - Platelet-rich plasma

KW - Scaffold

KW - TGF-β1

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