Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries

Francesco De Francesco, Virginia Tirino, Vincenzo Desiderio, Giuseppe Ferraro, Francesco D'Andrea, Mariateresa Giuliano, Guido Libondi, Giuseppe Pirozzi, Alfredo De Rosa, Gianpaolo Papaccio

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Background: Human adult adipose tissue is an abundant source of mesenchymal stem cells (MSCs). Moreover, it is an easily accessible site producing a considerable amount of stem cells. Methodology/Principal Findings: In this study, we have selected and characterized stem cells within the stromal vascular fraction (SVF) of human adult adipose tissue with the aim of understanding their differentiation capabilities and performance. We have found, within the SVF, different cell populations expressing MSC markers-including CD34, CD90, CD29, CD44, CD105, and CD117-and endothelial-progenitor-cell markers-including CD34, CD90, CD44, and CD54. Interestingly, CD34+/CD90+ cells formed sphere clusters, when placed in non-adherent growth conditions. Moreover, they showed a high proliferative capability, a telomerase activity that was significantly higher than that found in differentiated cells, and contained a fraction of cells displaying the phenotype of a side population. When cultured in adipogenic medium, CD34+/CD90+ quickly differentiated into adipocytes. In addition, they differentiated into endothelial cells (CD31+/VEGF+/Flk-1+) and, when placed in methylcellulose, were capable of forming capillary-like structures producing a high level of VEGF, as substantiated with ELISA tests. Conclusions/Significance: Our results demonstrate, for the first time, that CD34+/CD90+ cells of human adipose tissue are capable of forming sphere clusters, when grown in free-floating conditions, and differentiate in endothelial cells that form capillary-like structures in methylcellulose. These cells might be suitable for tissue reconstruction in regenerative medicine, especially when patients need treatments for vascular disease.

Original languageEnglish
Article numbere6537
JournalPLoS One
Volume4
Issue number8
DOIs
Publication statusPublished - Aug 6 2009

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Stem cells
Vascular Endothelial Growth Factor A
Endothelial cells
stem cells
Tissue
Methylcellulose
endothelial cells
adipose tissue
Adipose Tissue
methylcellulose
Cells
cells
Patient treatment
Mesenchymal Stromal Cells
blood vessels
Blood Vessels
Telomerase
Stem Cells
Endothelial Cells
telomerase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

De Francesco, F., Tirino, V., Desiderio, V., Ferraro, G., D'Andrea, F., Giuliano, M., ... Papaccio, G. (2009). Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One, 4(8), [e6537]. https://doi.org/10.1371/journal.pone.0006537

Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. / De Francesco, Francesco; Tirino, Virginia; Desiderio, Vincenzo; Ferraro, Giuseppe; D'Andrea, Francesco; Giuliano, Mariateresa; Libondi, Guido; Pirozzi, Giuseppe; De Rosa, Alfredo; Papaccio, Gianpaolo.

In: PLoS One, Vol. 4, No. 8, e6537, 06.08.2009.

Research output: Contribution to journalArticle

De Francesco, F, Tirino, V, Desiderio, V, Ferraro, G, D'Andrea, F, Giuliano, M, Libondi, G, Pirozzi, G, De Rosa, A & Papaccio, G 2009, 'Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries', PLoS One, vol. 4, no. 8, e6537. https://doi.org/10.1371/journal.pone.0006537
De Francesco, Francesco ; Tirino, Virginia ; Desiderio, Vincenzo ; Ferraro, Giuseppe ; D'Andrea, Francesco ; Giuliano, Mariateresa ; Libondi, Guido ; Pirozzi, Giuseppe ; De Rosa, Alfredo ; Papaccio, Gianpaolo. / Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. In: PLoS One. 2009 ; Vol. 4, No. 8.
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AU - Ferraro, Giuseppe

AU - D'Andrea, Francesco

AU - Giuliano, Mariateresa

AU - Libondi, Guido

AU - Pirozzi, Giuseppe

AU - De Rosa, Alfredo

AU - Papaccio, Gianpaolo

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