Human CD4 + T cell clone longevity in tissue culture: Lack of influence of donor age or cell origin

Graham Pawelec, Yvonne Barnett, Erminia Mariani, Rafael Solana

Research output: Contribution to journalArticle

Abstract

CD4 + human T cell clones were derived from activated peripheral blood lymphocytes of healthy young adults to establish cloning efficiencies (CE) and clonal longevities. These results were compared with those obtained using cells from the very elderly, also in excellent health. CE and both maximal and average longevities under appropriate culture conditions were very similar in the two groups. Moreover, CE of CD34 + hematopoietic progenitor cells and longevities of clones derived from them were also similar. Finally, CE and longevities of clones derived from a patient with chronic myelogenous leukaemia were found to be comparable as well. Hence, T cells with absolutely no antigenic exposure in vivo prior to cloning (i.e. CD34-derived) and those potentially exposed to chronic antigenic stimulation (CML-derived) and those from young or old donors all had similar cloning and propagation properties in vitro. These results imply that the longevity of T cells in culture is more likely to be dictated by cloning conditions than any intrinsic differences between the cells studied.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalExperimental Gerontology
Volume37
Issue number2-3
DOIs
Publication statusPublished - Jan 3 2002

Keywords

  • Donor age
  • Longevity
  • T cell clone
  • Tissue culture

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Fingerprint Dive into the research topics of 'Human CD4 + T cell clone longevity in tissue culture: Lack of influence of donor age or cell origin'. Together they form a unique fingerprint.

Cite this