Human CD4+ T cells specifically recognize a shared melanoma-associated antigen encoded by the tyrosinase gene

Suzanne L. Topalian, Licia Rivoltini, Marie Mancini, Nancy R. Markus, Paul F. Robbins, Yutaka Kawakami, Steven A. Rosenberg

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

Although commonly expressed human melanoma-associated antigens recognized by CD8+ cytolytic T cells have been described, little is known about CD4+ T-cell recognition of melanoma-associated antigens. Epstein-Barr virus- transformed B cells were used to present antigens derived from whole cell lysates of autologous and allogeneic melanomas for recognition by melanoma- specific CD4+ T-cell lines and clones cultured from tumor-infiltrating lymphocytes. HLA-DR-restricted antigens were detected in the lysates on the basis of specific release of cytokines from the responding T cells. Antigen sharing was demonstrated in the majority of melanomas tested, as well as in cultured normal melanocytes, but not in other normal tissues or nonmelanoma tumors. T-cell clones manifested a single recognition pattern, suggesting the presence of an immunodominant epitope. This epitope was identified as a product of the tyrosinase gene, which has also been shown to encode class I- restricted epitopes recognized by CD8+ T cells from melanoma patients. Identification of commonly expressed tumor-associated protein molecules containing epitopes presented by both class I and class II major histocompatibility molecules may provide optimal reagents for cancer immunization strategies.

Original languageEnglish
Pages (from-to)9461-9465
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number20
Publication statusPublished - Sep 27 1994

Fingerprint

Melanoma-Specific Antigens
Monophenol Monooxygenase
T-Lymphocytes
Melanoma
Genes
Epitopes
Clone Cells
Tumor-Infiltrating Lymphocytes
Antigens
Immunodominant Epitopes
Neoplasms
Histocompatibility
Melanocytes
HLA-DR Antigens
Human Herpesvirus 4
Immunization
B-Lymphocytes
Cytokines
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Human CD4+ T cells specifically recognize a shared melanoma-associated antigen encoded by the tyrosinase gene. / Topalian, Suzanne L.; Rivoltini, Licia; Mancini, Marie; Markus, Nancy R.; Robbins, Paul F.; Kawakami, Yutaka; Rosenberg, Steven A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 20, 27.09.1994, p. 9461-9465.

Research output: Contribution to journalArticle

Topalian, Suzanne L. ; Rivoltini, Licia ; Mancini, Marie ; Markus, Nancy R. ; Robbins, Paul F. ; Kawakami, Yutaka ; Rosenberg, Steven A. / Human CD4+ T cells specifically recognize a shared melanoma-associated antigen encoded by the tyrosinase gene. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 20. pp. 9461-9465.
@article{c004d800484649dea8373a356b3eed11,
title = "Human CD4+ T cells specifically recognize a shared melanoma-associated antigen encoded by the tyrosinase gene",
abstract = "Although commonly expressed human melanoma-associated antigens recognized by CD8+ cytolytic T cells have been described, little is known about CD4+ T-cell recognition of melanoma-associated antigens. Epstein-Barr virus- transformed B cells were used to present antigens derived from whole cell lysates of autologous and allogeneic melanomas for recognition by melanoma- specific CD4+ T-cell lines and clones cultured from tumor-infiltrating lymphocytes. HLA-DR-restricted antigens were detected in the lysates on the basis of specific release of cytokines from the responding T cells. Antigen sharing was demonstrated in the majority of melanomas tested, as well as in cultured normal melanocytes, but not in other normal tissues or nonmelanoma tumors. T-cell clones manifested a single recognition pattern, suggesting the presence of an immunodominant epitope. This epitope was identified as a product of the tyrosinase gene, which has also been shown to encode class I- restricted epitopes recognized by CD8+ T cells from melanoma patients. Identification of commonly expressed tumor-associated protein molecules containing epitopes presented by both class I and class II major histocompatibility molecules may provide optimal reagents for cancer immunization strategies.",
author = "Topalian, {Suzanne L.} and Licia Rivoltini and Marie Mancini and Markus, {Nancy R.} and Robbins, {Paul F.} and Yutaka Kawakami and Rosenberg, {Steven A.}",
year = "1994",
month = "9",
day = "27",
language = "English",
volume = "91",
pages = "9461--9465",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "20",

}

TY - JOUR

T1 - Human CD4+ T cells specifically recognize a shared melanoma-associated antigen encoded by the tyrosinase gene

AU - Topalian, Suzanne L.

AU - Rivoltini, Licia

AU - Mancini, Marie

AU - Markus, Nancy R.

AU - Robbins, Paul F.

AU - Kawakami, Yutaka

AU - Rosenberg, Steven A.

PY - 1994/9/27

Y1 - 1994/9/27

N2 - Although commonly expressed human melanoma-associated antigens recognized by CD8+ cytolytic T cells have been described, little is known about CD4+ T-cell recognition of melanoma-associated antigens. Epstein-Barr virus- transformed B cells were used to present antigens derived from whole cell lysates of autologous and allogeneic melanomas for recognition by melanoma- specific CD4+ T-cell lines and clones cultured from tumor-infiltrating lymphocytes. HLA-DR-restricted antigens were detected in the lysates on the basis of specific release of cytokines from the responding T cells. Antigen sharing was demonstrated in the majority of melanomas tested, as well as in cultured normal melanocytes, but not in other normal tissues or nonmelanoma tumors. T-cell clones manifested a single recognition pattern, suggesting the presence of an immunodominant epitope. This epitope was identified as a product of the tyrosinase gene, which has also been shown to encode class I- restricted epitopes recognized by CD8+ T cells from melanoma patients. Identification of commonly expressed tumor-associated protein molecules containing epitopes presented by both class I and class II major histocompatibility molecules may provide optimal reagents for cancer immunization strategies.

AB - Although commonly expressed human melanoma-associated antigens recognized by CD8+ cytolytic T cells have been described, little is known about CD4+ T-cell recognition of melanoma-associated antigens. Epstein-Barr virus- transformed B cells were used to present antigens derived from whole cell lysates of autologous and allogeneic melanomas for recognition by melanoma- specific CD4+ T-cell lines and clones cultured from tumor-infiltrating lymphocytes. HLA-DR-restricted antigens were detected in the lysates on the basis of specific release of cytokines from the responding T cells. Antigen sharing was demonstrated in the majority of melanomas tested, as well as in cultured normal melanocytes, but not in other normal tissues or nonmelanoma tumors. T-cell clones manifested a single recognition pattern, suggesting the presence of an immunodominant epitope. This epitope was identified as a product of the tyrosinase gene, which has also been shown to encode class I- restricted epitopes recognized by CD8+ T cells from melanoma patients. Identification of commonly expressed tumor-associated protein molecules containing epitopes presented by both class I and class II major histocompatibility molecules may provide optimal reagents for cancer immunization strategies.

UR - http://www.scopus.com/inward/record.url?scp=0028023727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028023727&partnerID=8YFLogxK

M3 - Article

VL - 91

SP - 9461

EP - 9465

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 20

ER -