Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold

Andrea Facchini, Gina Lisignoli, Sandra Cristino, Livia Roseti, Luciana De Franceschi, Emanuele Marconi, Brunella Grigolo

Research output: Contribution to journalArticle

Abstract

The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treatment of early lesions in osteoarthritis is under investigation. To obtain new information on the behaviour of chondrocytes and mesenchymal stem cells grown on a hyaluronan derivative scaffold (Hyaff®-11) already used in cartilage repair, we analysed a series of molecules expressed by these cells by Real-Time RT-PCR and immunohistochemical analyses. The data obtained with this work showed that this biomaterial is able to reduce the expression of some catabolic molecules by human chondrocytes and provide a good environment to support the differentiation of mesenchymal stem cells in chondrogenic sense. These observations confirm Hyaff®-11 as a suitable scaffold both for chondrocytes and mesenchymal stem cells for the treatment of articular cartilage defects.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalBiorheology
Volume43
Issue number3-4
Publication statusPublished - 2006

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Keywords

  • Chondrogenic differentiation
  • Human chondrocytes
  • Hyaluronan-based scaffold
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Facchini, A., Lisignoli, G., Cristino, S., Roseti, L., De Franceschi, L., Marconi, E., & Grigolo, B. (2006). Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold. Biorheology, 43(3-4), 471-480.