Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold

Andrea Facchini, Gina Lisignoli, Sandra Cristino, Livia Roseti, Luciana De Franceschi, Emanuele Marconi, Brunella Grigolo

Research output: Contribution to journalArticle

Abstract

The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treatment of early lesions in osteoarthritis is under investigation. To obtain new information on the behaviour of chondrocytes and mesenchymal stem cells grown on a hyaluronan derivative scaffold (Hyaff®-11) already used in cartilage repair, we analysed a series of molecules expressed by these cells by Real-Time RT-PCR and immunohistochemical analyses. The data obtained with this work showed that this biomaterial is able to reduce the expression of some catabolic molecules by human chondrocytes and provide a good environment to support the differentiation of mesenchymal stem cells in chondrogenic sense. These observations confirm Hyaff®-11 as a suitable scaffold both for chondrocytes and mesenchymal stem cells for the treatment of articular cartilage defects.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalBiorheology
Volume43
Issue number3-4
Publication statusPublished - 2006

Fingerprint

Chondrocytes
Mesenchymal Stromal Cells
Cartilage
Biocompatible Materials
Articular Cartilage
Hyaluronic Acid
Osteoarthritis
Real-Time Polymerase Chain Reaction
Equipment and Supplies

Keywords

  • Chondrogenic differentiation
  • Human chondrocytes
  • Hyaluronan-based scaffold
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Facchini, A., Lisignoli, G., Cristino, S., Roseti, L., De Franceschi, L., Marconi, E., & Grigolo, B. (2006). Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold. Biorheology, 43(3-4), 471-480.

Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold. / Facchini, Andrea; Lisignoli, Gina; Cristino, Sandra; Roseti, Livia; De Franceschi, Luciana; Marconi, Emanuele; Grigolo, Brunella.

In: Biorheology, Vol. 43, No. 3-4, 2006, p. 471-480.

Research output: Contribution to journalArticle

Facchini, A, Lisignoli, G, Cristino, S, Roseti, L, De Franceschi, L, Marconi, E & Grigolo, B 2006, 'Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold', Biorheology, vol. 43, no. 3-4, pp. 471-480.
Facchini A, Lisignoli G, Cristino S, Roseti L, De Franceschi L, Marconi E et al. Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold. Biorheology. 2006;43(3-4):471-480.
Facchini, Andrea ; Lisignoli, Gina ; Cristino, Sandra ; Roseti, Livia ; De Franceschi, Luciana ; Marconi, Emanuele ; Grigolo, Brunella. / Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold. In: Biorheology. 2006 ; Vol. 43, No. 3-4. pp. 471-480.
@article{6266d630ae54492884871b9d520efe49,
title = "Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold",
abstract = "The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treatment of early lesions in osteoarthritis is under investigation. To obtain new information on the behaviour of chondrocytes and mesenchymal stem cells grown on a hyaluronan derivative scaffold (Hyaff{\circledR}-11) already used in cartilage repair, we analysed a series of molecules expressed by these cells by Real-Time RT-PCR and immunohistochemical analyses. The data obtained with this work showed that this biomaterial is able to reduce the expression of some catabolic molecules by human chondrocytes and provide a good environment to support the differentiation of mesenchymal stem cells in chondrogenic sense. These observations confirm Hyaff{\circledR}-11 as a suitable scaffold both for chondrocytes and mesenchymal stem cells for the treatment of articular cartilage defects.",
keywords = "Chondrogenic differentiation, Human chondrocytes, Hyaluronan-based scaffold, Mesenchymal stem cells",
author = "Andrea Facchini and Gina Lisignoli and Sandra Cristino and Livia Roseti and {De Franceschi}, Luciana and Emanuele Marconi and Brunella Grigolo",
year = "2006",
language = "English",
volume = "43",
pages = "471--480",
journal = "Biorheology",
issn = "0006-355X",
publisher = "IOS Press",
number = "3-4",

}

TY - JOUR

T1 - Human chondrocytes and mesenchymal stem cells grown onto engineered scaffold

AU - Facchini, Andrea

AU - Lisignoli, Gina

AU - Cristino, Sandra

AU - Roseti, Livia

AU - De Franceschi, Luciana

AU - Marconi, Emanuele

AU - Grigolo, Brunella

PY - 2006

Y1 - 2006

N2 - The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treatment of early lesions in osteoarthritis is under investigation. To obtain new information on the behaviour of chondrocytes and mesenchymal stem cells grown on a hyaluronan derivative scaffold (Hyaff®-11) already used in cartilage repair, we analysed a series of molecules expressed by these cells by Real-Time RT-PCR and immunohistochemical analyses. The data obtained with this work showed that this biomaterial is able to reduce the expression of some catabolic molecules by human chondrocytes and provide a good environment to support the differentiation of mesenchymal stem cells in chondrogenic sense. These observations confirm Hyaff®-11 as a suitable scaffold both for chondrocytes and mesenchymal stem cells for the treatment of articular cartilage defects.

AB - The development of improved methods for treatment of chondral defects using autologous cells in combination with biomaterials leads to a new generation of implantable devices. Their association gives rise to a hybrid construct combining biological and material components that can be specifically committed. The comprehension of cellular and molecular mechanisms of cartilage repair and the use of biomaterials in combination with chondrocytes or mesenchymal stem cells in the treatment of cartilage defects has opened a new era of therapeutical strategies. Recently, their applicability in the treatment of early lesions in osteoarthritis is under investigation. To obtain new information on the behaviour of chondrocytes and mesenchymal stem cells grown on a hyaluronan derivative scaffold (Hyaff®-11) already used in cartilage repair, we analysed a series of molecules expressed by these cells by Real-Time RT-PCR and immunohistochemical analyses. The data obtained with this work showed that this biomaterial is able to reduce the expression of some catabolic molecules by human chondrocytes and provide a good environment to support the differentiation of mesenchymal stem cells in chondrogenic sense. These observations confirm Hyaff®-11 as a suitable scaffold both for chondrocytes and mesenchymal stem cells for the treatment of articular cartilage defects.

KW - Chondrogenic differentiation

KW - Human chondrocytes

KW - Hyaluronan-based scaffold

KW - Mesenchymal stem cells

UR - http://www.scopus.com/inward/record.url?scp=33747136885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747136885&partnerID=8YFLogxK

M3 - Article

C2 - 16912418

AN - SCOPUS:33747136885

VL - 43

SP - 471

EP - 480

JO - Biorheology

JF - Biorheology

SN - 0006-355X

IS - 3-4

ER -