Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies

Marco Denegri, Daniela Moralli, Mariano Rocchi, Marco Biggiogera, Elena Raimondi, Fabio Cobianchi, Luigi De Carli, Silvano Riva, Giuseppe Biamonti

Research output: Contribution to journalArticle

Abstract

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP Al-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Original languageEnglish
Pages (from-to)2069-2079
Number of pages11
JournalMolecular Biology of the Cell
Volume13
Issue number6
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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    Denegri, M., Moralli, D., Rocchi, M., Biggiogera, M., Raimondi, E., Cobianchi, F., De Carli, L., Riva, S., & Biamonti, G. (2002). Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies. Molecular Biology of the Cell, 13(6), 2069-2079. https://doi.org/10.1091/mbc.01-12-0569