Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies

Marco Denegri, Daniela Moralli, Mariano Rocchi, Marco Biggiogera, Elena Raimondi, Fabio Cobianchi, Luigi De Carli, Silvano Riva, Giuseppe Biamonti

Research output: Contribution to journalArticle

Abstract

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP Al-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Original languageEnglish
Pages (from-to)2069-2079
Number of pages11
JournalMolecular Biology of the Cell
Volume13
Issue number6
DOIs
Publication statusPublished - 2002

Fingerprint

Chromosomes, Human, Pair 15
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 9
Human Chromosomes
Cricetinae
Hybrid Cells
Hot Temperature
HeLa Cells
Heterogeneous-Nuclear Ribonucleoproteins
Heat-Shock Proteins
Fluorescence In Situ Hybridization
Mitosis
Shock
RNA

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Denegri, M., Moralli, D., Rocchi, M., Biggiogera, M., Raimondi, E., Cobianchi, F., ... Biamonti, G. (2002). Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies. Molecular Biology of the Cell, 13(6), 2069-2079. https://doi.org/10.1091/mbc.01-12-0569

Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies. / Denegri, Marco; Moralli, Daniela; Rocchi, Mariano; Biggiogera, Marco; Raimondi, Elena; Cobianchi, Fabio; De Carli, Luigi; Riva, Silvano; Biamonti, Giuseppe.

In: Molecular Biology of the Cell, Vol. 13, No. 6, 2002, p. 2069-2079.

Research output: Contribution to journalArticle

Denegri, M, Moralli, D, Rocchi, M, Biggiogera, M, Raimondi, E, Cobianchi, F, De Carli, L, Riva, S & Biamonti, G 2002, 'Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies', Molecular Biology of the Cell, vol. 13, no. 6, pp. 2069-2079. https://doi.org/10.1091/mbc.01-12-0569
Denegri, Marco ; Moralli, Daniela ; Rocchi, Mariano ; Biggiogera, Marco ; Raimondi, Elena ; Cobianchi, Fabio ; De Carli, Luigi ; Riva, Silvano ; Biamonti, Giuseppe. / Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 6. pp. 2069-2079.
@article{f6934c6eada84f02a96126f07dc57a36,
title = "Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies",
abstract = "We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP Al-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.",
author = "Marco Denegri and Daniela Moralli and Mariano Rocchi and Marco Biggiogera and Elena Raimondi and Fabio Cobianchi and {De Carli}, Luigi and Silvano Riva and Giuseppe Biamonti",
year = "2002",
doi = "10.1091/mbc.01-12-0569",
language = "English",
volume = "13",
pages = "2069--2079",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "6",

}

TY - JOUR

T1 - Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies

AU - Denegri, Marco

AU - Moralli, Daniela

AU - Rocchi, Mariano

AU - Biggiogera, Marco

AU - Raimondi, Elena

AU - Cobianchi, Fabio

AU - De Carli, Luigi

AU - Riva, Silvano

AU - Biamonti, Giuseppe

PY - 2002

Y1 - 2002

N2 - We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP Al-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

AB - We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP Al-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

UR - http://www.scopus.com/inward/record.url?scp=0035985201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035985201&partnerID=8YFLogxK

U2 - 10.1091/mbc.01-12-0569

DO - 10.1091/mbc.01-12-0569

M3 - Article

C2 - 12058070

AN - SCOPUS:0035985201

VL - 13

SP - 2069

EP - 2079

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 6

ER -