Human cord blood CD1331 stem cells transplanted to nod-scid mice provide conditions for regeneration of olfactory neuroepithelium after permanent damage induced by dichlobenil

Valeria Franceschini, Simone Bettini, Simone Pifferi, Alfredo Rosellini, Anna Menini, Ricardo Saccardi, Emanuela Ognio, Rosemary Jeffery, Richard Poulsom, Roberto P. Revoltella

Research output: Contribution to journalArticle

Abstract

The herbicide dichlobenil selectively causes necrosis of the dorsomedial part of olfactory neuroepithelium (NE) with permanent damage to the underlying mucosa, whereas the lateral part of the olfactory region and the nasal respiratory mucosa remain undamaged. We investigated here whether human umbilical cord blood CD1331 stem cells (HSC) injected intravenously to nod-scid mice pretreated with dichlobenil may engraft the olfactory mucosa and contribute to the regeneration of the damaged NE. We tested HLA-DQa1 DNA and three human microsatellites (Combined DNA Index System) as indicators of engrafted cells, finding polymerase chain reaction evidence of chimaerism in various tissues of the host, including the olfactory mucosa and bulb, at 7 and 31 days following HSC transplantation. Histology, immunohistochemistry, and lectin staining revealed the morphological recovery of the dorsomedial region of the NE in dichlobenil-treated mice that received HSC, contrasting with the lack of regeneration in similarly injured areas as these remained damaged in control nontransplanted mice. FISH analysis, to detect human genomic sequences from different chromosomes, confirmed persistent engraftment of the regenerating olfactory area with chimeric cells. Electro-olfactograms in response to odorants, to test the functionality of the olfactory NE, confirmed the functional damage of the dorsomedial area in dichlobenil- treated mice and the functional recovery of the same area in transplanted mice. These findings support the concept that transplanted HSC migrating to the damaged olfactory area provide conditions facilitating the recovery from olfactory receptor cell loss.

Original languageEnglish
Pages (from-to)825-835
Number of pages11
JournalStem Cells
Volume27
Issue number4
DOIs
Publication statusPublished - Apr 1 2009

Fingerprint

Fetal Blood
Regeneration
Stem Cells
Olfactory Mucosa
Olfactory Receptor Neurons
Respiratory Mucosa
Nasal Mucosa
Olfactory Bulb
DNA
Herbicides
Lectins
Microsatellite Repeats
Histology
Mucous Membrane
Necrosis
Chromosomes
Transplantation
Immunohistochemistry
dichlobanil
Staining and Labeling

Keywords

  • CD1331 stem cells
  • Chimaerism
  • Cord blood
  • Dichlobenil
  • Olfactory neuroepithelium
  • Olfactory tissue regeneration

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Cite this

Human cord blood CD1331 stem cells transplanted to nod-scid mice provide conditions for regeneration of olfactory neuroepithelium after permanent damage induced by dichlobenil. / Franceschini, Valeria; Bettini, Simone; Pifferi, Simone; Rosellini, Alfredo; Menini, Anna; Saccardi, Ricardo; Ognio, Emanuela; Jeffery, Rosemary; Poulsom, Richard; Revoltella, Roberto P.

In: Stem Cells, Vol. 27, No. 4, 01.04.2009, p. 825-835.

Research output: Contribution to journalArticle

Franceschini, V, Bettini, S, Pifferi, S, Rosellini, A, Menini, A, Saccardi, R, Ognio, E, Jeffery, R, Poulsom, R & Revoltella, RP 2009, 'Human cord blood CD1331 stem cells transplanted to nod-scid mice provide conditions for regeneration of olfactory neuroepithelium after permanent damage induced by dichlobenil', Stem Cells, vol. 27, no. 4, pp. 825-835. https://doi.org/10.1002/stem.11
Franceschini, Valeria ; Bettini, Simone ; Pifferi, Simone ; Rosellini, Alfredo ; Menini, Anna ; Saccardi, Ricardo ; Ognio, Emanuela ; Jeffery, Rosemary ; Poulsom, Richard ; Revoltella, Roberto P. / Human cord blood CD1331 stem cells transplanted to nod-scid mice provide conditions for regeneration of olfactory neuroepithelium after permanent damage induced by dichlobenil. In: Stem Cells. 2009 ; Vol. 27, No. 4. pp. 825-835.
@article{9b694dac9d724ecb952583588d8249c9,
title = "Human cord blood CD1331 stem cells transplanted to nod-scid mice provide conditions for regeneration of olfactory neuroepithelium after permanent damage induced by dichlobenil",
abstract = "The herbicide dichlobenil selectively causes necrosis of the dorsomedial part of olfactory neuroepithelium (NE) with permanent damage to the underlying mucosa, whereas the lateral part of the olfactory region and the nasal respiratory mucosa remain undamaged. We investigated here whether human umbilical cord blood CD1331 stem cells (HSC) injected intravenously to nod-scid mice pretreated with dichlobenil may engraft the olfactory mucosa and contribute to the regeneration of the damaged NE. We tested HLA-DQa1 DNA and three human microsatellites (Combined DNA Index System) as indicators of engrafted cells, finding polymerase chain reaction evidence of chimaerism in various tissues of the host, including the olfactory mucosa and bulb, at 7 and 31 days following HSC transplantation. Histology, immunohistochemistry, and lectin staining revealed the morphological recovery of the dorsomedial region of the NE in dichlobenil-treated mice that received HSC, contrasting with the lack of regeneration in similarly injured areas as these remained damaged in control nontransplanted mice. FISH analysis, to detect human genomic sequences from different chromosomes, confirmed persistent engraftment of the regenerating olfactory area with chimeric cells. Electro-olfactograms in response to odorants, to test the functionality of the olfactory NE, confirmed the functional damage of the dorsomedial area in dichlobenil- treated mice and the functional recovery of the same area in transplanted mice. These findings support the concept that transplanted HSC migrating to the damaged olfactory area provide conditions facilitating the recovery from olfactory receptor cell loss.",
keywords = "CD1331 stem cells, Chimaerism, Cord blood, Dichlobenil, Olfactory neuroepithelium, Olfactory tissue regeneration",
author = "Valeria Franceschini and Simone Bettini and Simone Pifferi and Alfredo Rosellini and Anna Menini and Ricardo Saccardi and Emanuela Ognio and Rosemary Jeffery and Richard Poulsom and Revoltella, {Roberto P.}",
year = "2009",
month = "4",
day = "1",
doi = "10.1002/stem.11",
language = "English",
volume = "27",
pages = "825--835",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "4",

}

TY - JOUR

T1 - Human cord blood CD1331 stem cells transplanted to nod-scid mice provide conditions for regeneration of olfactory neuroepithelium after permanent damage induced by dichlobenil

AU - Franceschini, Valeria

AU - Bettini, Simone

AU - Pifferi, Simone

AU - Rosellini, Alfredo

AU - Menini, Anna

AU - Saccardi, Ricardo

AU - Ognio, Emanuela

AU - Jeffery, Rosemary

AU - Poulsom, Richard

AU - Revoltella, Roberto P.

PY - 2009/4/1

Y1 - 2009/4/1

N2 - The herbicide dichlobenil selectively causes necrosis of the dorsomedial part of olfactory neuroepithelium (NE) with permanent damage to the underlying mucosa, whereas the lateral part of the olfactory region and the nasal respiratory mucosa remain undamaged. We investigated here whether human umbilical cord blood CD1331 stem cells (HSC) injected intravenously to nod-scid mice pretreated with dichlobenil may engraft the olfactory mucosa and contribute to the regeneration of the damaged NE. We tested HLA-DQa1 DNA and three human microsatellites (Combined DNA Index System) as indicators of engrafted cells, finding polymerase chain reaction evidence of chimaerism in various tissues of the host, including the olfactory mucosa and bulb, at 7 and 31 days following HSC transplantation. Histology, immunohistochemistry, and lectin staining revealed the morphological recovery of the dorsomedial region of the NE in dichlobenil-treated mice that received HSC, contrasting with the lack of regeneration in similarly injured areas as these remained damaged in control nontransplanted mice. FISH analysis, to detect human genomic sequences from different chromosomes, confirmed persistent engraftment of the regenerating olfactory area with chimeric cells. Electro-olfactograms in response to odorants, to test the functionality of the olfactory NE, confirmed the functional damage of the dorsomedial area in dichlobenil- treated mice and the functional recovery of the same area in transplanted mice. These findings support the concept that transplanted HSC migrating to the damaged olfactory area provide conditions facilitating the recovery from olfactory receptor cell loss.

AB - The herbicide dichlobenil selectively causes necrosis of the dorsomedial part of olfactory neuroepithelium (NE) with permanent damage to the underlying mucosa, whereas the lateral part of the olfactory region and the nasal respiratory mucosa remain undamaged. We investigated here whether human umbilical cord blood CD1331 stem cells (HSC) injected intravenously to nod-scid mice pretreated with dichlobenil may engraft the olfactory mucosa and contribute to the regeneration of the damaged NE. We tested HLA-DQa1 DNA and three human microsatellites (Combined DNA Index System) as indicators of engrafted cells, finding polymerase chain reaction evidence of chimaerism in various tissues of the host, including the olfactory mucosa and bulb, at 7 and 31 days following HSC transplantation. Histology, immunohistochemistry, and lectin staining revealed the morphological recovery of the dorsomedial region of the NE in dichlobenil-treated mice that received HSC, contrasting with the lack of regeneration in similarly injured areas as these remained damaged in control nontransplanted mice. FISH analysis, to detect human genomic sequences from different chromosomes, confirmed persistent engraftment of the regenerating olfactory area with chimeric cells. Electro-olfactograms in response to odorants, to test the functionality of the olfactory NE, confirmed the functional damage of the dorsomedial area in dichlobenil- treated mice and the functional recovery of the same area in transplanted mice. These findings support the concept that transplanted HSC migrating to the damaged olfactory area provide conditions facilitating the recovery from olfactory receptor cell loss.

KW - CD1331 stem cells

KW - Chimaerism

KW - Cord blood

KW - Dichlobenil

KW - Olfactory neuroepithelium

KW - Olfactory tissue regeneration

UR - http://www.scopus.com/inward/record.url?scp=65649153639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65649153639&partnerID=8YFLogxK

U2 - 10.1002/stem.11

DO - 10.1002/stem.11

M3 - Article

C2 - 19350683

AN - SCOPUS:65649153639

VL - 27

SP - 825

EP - 835

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 4

ER -