Human coronavirus OC43 serum inhibitor and neutralizing antibody by a new plaque-reduction assay

G. Gerna, E. Cattaneo, P. M. Cereda, M. G. Revelo, G. Achilli

Research output: Contribution to journalArticlepeer-review

Abstract

A plaque-reduction assay for the determination of neutralizing antibody to human coronavirus OC43 has been set up, using as a plaquing factor the fetal calf serum containing an OC43 inhibitor. A similar inhibitor has also been found in human sera both negative and positive for OC43 antibody. The inhibitor can be removed by treatment of sera with phospholipase C and is likely to be a lipoprotein of the high density class. The new plaque-reduction assay appears to be a very sensitive test for both OC43 antibody determination and serodiagnosis of OC43 infections. It may also be very useful for studies of antigenic relationships between human and animal coronaviruses. However, before testing, sera must be routinely treated for removal of viral inhibitor.

Original languageEnglish
Pages (from-to)360-366
Number of pages7
JournalProceedings of the Society for Experimental Biology and Medicine
Volume163
Issue number3
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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