Human cytomegalovirus infection promotes rapid maturation of NK cells expressing activating killer Ig-like receptor in patients transplanted with NKG2C-/- umbilical cord blood

Mariella Della Chiesa, Michela Falco, Alice Bertaina, Letizia Muccio, Claudia Alicata, Francesco Frassoni, Franco Locatelli, Lorenzo Moretta, Alessandro Moretta

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Abstract

NK cells are the first lymphoid population recovering after allogeneic hematopoietic stem cell transplantation and play a crucial role in early immunity after the graft. Recently, it has been shown that humanCMV (HCMV) infection/reactivation can deeply influence NK cell reconstitution after umbilical cord blood transplantation by accelerating the differentiation of mature NKG2A-killer Ig-like receptor (KIR)+ NK cells characterized by the expression of the NKG2C-activating receptor. In view of the hypothesis that NKG2C could be directly involved in NK cell maturation driven by HCMV infection, we analyzed the maturation and function of NK cells developing in three patients with hematological malignancies given umbilical cord blood transplantation from donors carrying a homozygous deletion of the NKG2C gene. We show that HCMV infection can drive rapid NK maturation, characterized by the expansion of CD56dimNKG2A-KIR + cells, even in the absence of NKG2C expression. Interestingly, this expanded mature NK cell subset expressed surface-activating KIR that could trigger NK cell cytotoxicity, degranulation, and IFN-γ release. Given the absence of NKG2C, it is conceivable that activating KIRs may play a role in the HCMV-driven NK cell maturation and that NK cells expressing activating KIRs might contribute, at least in part, to the control of infections after transplantation.

Original languageEnglish
Pages (from-to)1471-1479
Number of pages9
JournalJournal of Immunology
Volume192
Issue number4
DOIs
Publication statusPublished - Feb 15 2014

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Cytomegalovirus Infections
Fetal Blood
Natural Killer Cells
Transplantation
NK Cell Lectin-Like Receptor Subfamily C
Infection
Natural Killer Cell Receptors
Cell Degranulation
Hematopoietic Stem Cell Transplantation
Gene Deletion
Hematologic Neoplasms
Infection Control
Blood Donors
Immunity
Transplants
Population

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Human cytomegalovirus infection promotes rapid maturation of NK cells expressing activating killer Ig-like receptor in patients transplanted with NKG2C-/- umbilical cord blood",
abstract = "NK cells are the first lymphoid population recovering after allogeneic hematopoietic stem cell transplantation and play a crucial role in early immunity after the graft. Recently, it has been shown that humanCMV (HCMV) infection/reactivation can deeply influence NK cell reconstitution after umbilical cord blood transplantation by accelerating the differentiation of mature NKG2A-killer Ig-like receptor (KIR)+ NK cells characterized by the expression of the NKG2C-activating receptor. In view of the hypothesis that NKG2C could be directly involved in NK cell maturation driven by HCMV infection, we analyzed the maturation and function of NK cells developing in three patients with hematological malignancies given umbilical cord blood transplantation from donors carrying a homozygous deletion of the NKG2C gene. We show that HCMV infection can drive rapid NK maturation, characterized by the expansion of CD56dimNKG2A-KIR + cells, even in the absence of NKG2C expression. Interestingly, this expanded mature NK cell subset expressed surface-activating KIR that could trigger NK cell cytotoxicity, degranulation, and IFN-γ release. Given the absence of NKG2C, it is conceivable that activating KIRs may play a role in the HCMV-driven NK cell maturation and that NK cells expressing activating KIRs might contribute, at least in part, to the control of infections after transplantation.",
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T1 - Human cytomegalovirus infection promotes rapid maturation of NK cells expressing activating killer Ig-like receptor in patients transplanted with NKG2C-/- umbilical cord blood

AU - Della Chiesa, Mariella

AU - Falco, Michela

AU - Bertaina, Alice

AU - Muccio, Letizia

AU - Alicata, Claudia

AU - Frassoni, Francesco

AU - Locatelli, Franco

AU - Moretta, Lorenzo

AU - Moretta, Alessandro

PY - 2014/2/15

Y1 - 2014/2/15

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