Human cytomegalovirus-specific CD4+ and CD8+ T-cell response determination: Comparison of short-term (24h) assays vs long-term (7-day) infected dendritic cell assay in the immunocompetent and the immunocompromised host

Paola Zelini, Daniele Lilleri, Giuditta Comolli, Vanina Rognoni, Antonella Chiesa, Chiara Fornara, Franco Locatelli, Federica Meloni, Giuseppe Gerna

Research output: Contribution to journalArticlepeer-review

Abstract

Human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T-cells were measured in the immunocompetent host as well as in 13 solid-organ transplant recipients (SOTR), and 12 young hematopoietic stem cell transplant recipients (HSCTR) by using a long-term (7-day) assay based on PBMC stimulation by HCMV-infected dendritic cells (iDC), and two short-term (24h) assays, one for CD4+ stimulation by infected cell lysate (iCL), and the other for CD8+ stimulation by a pool of 34 epitopic peptides (pep-pool). In the immunocompetent, the number of T-cells activated by either iCL or the pep-pool was significantly reduced with respect to iDC. In both SOTR and HSCTR, the number of T-cells activated by iDC was comparable to that activated by iCL or the pep-pool. A significant correlation between iDC-activated T-cells and T-cells activated by either iCL or the pep-pool was observed. In conclusion, whenever a rapid result is needed, short-term assays may efficiently replace the iDC assay.

Original languageEnglish
Pages (from-to)269-281
Number of pages13
JournalClinical Immunology
Volume136
Issue number2
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Epitopic peptide pool
  • Human cytomegalovirus
  • Immunocompetent host
  • Immunocompromised patients
  • Infected cell lysate
  • Infected dendritic cells
  • T-cell response

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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