Abstract
Background. Reconstitution of human cytomegalovirus (HCMV) T cell-specific immunity is of crucial relevance in patients receiving a hematopoietic stem cell transplant (HSCT). Scarce data on this subject are available for children receiving a T cell- depleted HSCT. Methods. We investigated HCMV-specific T cell recovery in 48 recipients of a T cell- depleted HSCT from a human leukocyte antigen (HLA)-disparate relative. Autologous HCMV-infected dendritic cells were used to stimulate HCMV-specific CD8 + and CD4 + T cells producing interferon-γ (IFN). Results. The 1-year cumulative incidence of both HCMV infection and specific T cell reconstitution was 83% among the 23 HCMV-seropositive patients and 4% and 8%, respectively, among the 25 HCMV-seronegative patients (P <.01). HCMV-specific T cell reconstitution was significantly delayed in patients receiving T cell- depleted grafts, compared with patients receiving unmanipulated HSCTs (median time to reconstitution, 75 vs. 47 days, respectively; P <.01). The median time from HCMV infection to immune recovery in recipients of T cell- depleted grafts was 47 days. Detection of HCMV-specific T cells correlated with control of HCMV infection. The number of residual T cells in the graft predicted earlier T cell recovery (P = .02). Conclusions. Latent HCMV in the recipient was the major cause of HCMV reactivation and also promoted specific T cell reconstitution in patients given a T cell- depleted HSCT from an HLA-disparate relative. Routine immunologic monitoring is valuable in identifying patients with early HCMV-specific T cell reconstitution.
Original language | English |
---|---|
Pages (from-to) | 829-836 |
Number of pages | 8 |
Journal | Journal of Infectious Diseases |
Volume | 199 |
Issue number | 6 |
DOIs | |
Publication status | Published - Mar 15 2009 |
ASJC Scopus subject areas
- Infectious Diseases
- Immunology and Allergy