Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii

Silvia Corinti, Donata Medaglini, Caterina Prezzi, Andrea Cavani, Gianni Pozzi, Giampiero Girolomoni

Research output: Contribution to journalArticle

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Abstract

Bacteria are being actively investigated as vaccine carriers for inducing or boosting protective immune responses. In this study, human monocyte-derived dendritic cells (DCs) and normal B cells were compared for their capacity to present the C fragment of tetanus toxin (TTFC), expressed on the surface of recombinant Streptococcus gordonii, to specific CD4+ T lymphocytes. DCs were more efficient than B cells at presenting soluble TTFC and remarkably more capable of presenting bacterium-associated TTFC both in terms of the amount of antigen required to obtain a given T-cell response and on a per-cell basis. This difference was associated with a much lower capacity of B cells to endocytose soluble TTFC and phagocytose recombinant S. gordonii. In addition, S. gordonii induced the phenotypic maturation of DCs but not of B cells. The results thus indicate that DCs but not B cells play a crucial role in the amplification of class II-restricted immune responses induced by immunization with recombinant gram-positive bacteria.

Original languageEnglish
Pages (from-to)1879-1883
Number of pages5
JournalInfection and Immunity
Volume68
Issue number4
DOIs
Publication statusPublished - Apr 2000

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Streptococcus gordonii
Heterophile Antigens
Major Histocompatibility Complex
Dendritic Cells
B-Lymphocytes
Bacteria
T-Lymphocytes
Gram-Positive Bacteria
Endocytosis
Phagocytosis
Monocytes
Immunization
Vaccines
Antigens

ASJC Scopus subject areas

  • Immunology

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Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii. / Corinti, Silvia; Medaglini, Donata; Prezzi, Caterina; Cavani, Andrea; Pozzi, Gianni; Girolomoni, Giampiero.

In: Infection and Immunity, Vol. 68, No. 4, 04.2000, p. 1879-1883.

Research output: Contribution to journalArticle

Corinti, Silvia ; Medaglini, Donata ; Prezzi, Caterina ; Cavani, Andrea ; Pozzi, Gianni ; Girolomoni, Giampiero. / Human dendritic cells are superior to B cells at presenting a major histocompatibility complex class II-restricted heterologous antigen expressed on recombinant Streptococcus gordonii. In: Infection and Immunity. 2000 ; Vol. 68, No. 4. pp. 1879-1883.
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