Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration

G. C. Manara, G. Pasquinelli, L. Badiali-De Giorgi, C. Ferrari, S. A. Garatti, D. Fasano, E. Berti

Research output: Contribution to journalArticle

Abstract

Three ligands have been described for the leucocyte integrin LFA-1, namely intercellular adhesion molecule (ICAM)-1; ICAM-2, and ICAM-3. ICAMs show differences in tissue distribution and inducibility. The recently described ICAM-3 is highly expressed on resting lymphocytes, monocytes and neutrophils. Here, we demonstrate that the whole human epidermal Langerhans cell (LC) population expresses this molecule. Immunohistochemical staining of skin sections with an anti-ICAM-3 monoclonal antibody displayed reactivity with dendritic epidermal cells regularly distributed along the epidermis. Highly-sensitive immunoelectron microscopy procedures, performed on freshly suspended epidermal cells both at transmission and scanning electron microscopic levels, enabled demonstration that the whole LC population expresses cell surface ICAM-3. In contrast, keratinocytes and melanocytes were consistently negative. The prominent expression of ICAM-3 by resident LC could imply a crucial part for this molecule in leucocyte-intercellular interactions in the skin.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalBritish Journal of Dermatology
Volume134
Issue number1
Publication statusPublished - 1996

Fingerprint

Langerhans Cells
Cell Adhesion Molecules
Leukocytes
Lymphocyte Function-Associated Antigen-1
Skin
Immunoelectron Microscopy
Melanocytes
Tissue Distribution
Intercellular Adhesion Molecule-1
Keratinocytes
Epidermis
Integrins
Population
Monocytes
Neutrophils
Monoclonal Antibodies
Lymphocytes
Electrons
Staining and Labeling
Ligands

ASJC Scopus subject areas

  • Dermatology

Cite this

Manara, G. C., Pasquinelli, G., Badiali-De Giorgi, L., Ferrari, C., Garatti, S. A., Fasano, D., & Berti, E. (1996). Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration. British Journal of Dermatology, 134(1), 22-27.

Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration. / Manara, G. C.; Pasquinelli, G.; Badiali-De Giorgi, L.; Ferrari, C.; Garatti, S. A.; Fasano, D.; Berti, E.

In: British Journal of Dermatology, Vol. 134, No. 1, 1996, p. 22-27.

Research output: Contribution to journalArticle

Manara, GC, Pasquinelli, G, Badiali-De Giorgi, L, Ferrari, C, Garatti, SA, Fasano, D & Berti, E 1996, 'Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration', British Journal of Dermatology, vol. 134, no. 1, pp. 22-27.
Manara GC, Pasquinelli G, Badiali-De Giorgi L, Ferrari C, Garatti SA, Fasano D et al. Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration. British Journal of Dermatology. 1996;134(1):22-27.
Manara, G. C. ; Pasquinelli, G. ; Badiali-De Giorgi, L. ; Ferrari, C. ; Garatti, S. A. ; Fasano, D. ; Berti, E. / Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration. In: British Journal of Dermatology. 1996 ; Vol. 134, No. 1. pp. 22-27.
@article{42d87c0058e14ba5942d14df36b5c4ed,
title = "Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration",
abstract = "Three ligands have been described for the leucocyte integrin LFA-1, namely intercellular adhesion molecule (ICAM)-1; ICAM-2, and ICAM-3. ICAMs show differences in tissue distribution and inducibility. The recently described ICAM-3 is highly expressed on resting lymphocytes, monocytes and neutrophils. Here, we demonstrate that the whole human epidermal Langerhans cell (LC) population expresses this molecule. Immunohistochemical staining of skin sections with an anti-ICAM-3 monoclonal antibody displayed reactivity with dendritic epidermal cells regularly distributed along the epidermis. Highly-sensitive immunoelectron microscopy procedures, performed on freshly suspended epidermal cells both at transmission and scanning electron microscopic levels, enabled demonstration that the whole LC population expresses cell surface ICAM-3. In contrast, keratinocytes and melanocytes were consistently negative. The prominent expression of ICAM-3 by resident LC could imply a crucial part for this molecule in leucocyte-intercellular interactions in the skin.",
author = "Manara, {G. C.} and G. Pasquinelli and {Badiali-De Giorgi}, L. and C. Ferrari and Garatti, {S. A.} and D. Fasano and E. Berti",
year = "1996",
language = "English",
volume = "134",
pages = "22--27",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Blackwell Publishing Ltd",
number = "1",

}

TY - JOUR

T1 - Human epidermal Langerhans cells express the ICAM-3 molecule. Immunohistochemical and immunoelectron microscopical demonstration

AU - Manara, G. C.

AU - Pasquinelli, G.

AU - Badiali-De Giorgi, L.

AU - Ferrari, C.

AU - Garatti, S. A.

AU - Fasano, D.

AU - Berti, E.

PY - 1996

Y1 - 1996

N2 - Three ligands have been described for the leucocyte integrin LFA-1, namely intercellular adhesion molecule (ICAM)-1; ICAM-2, and ICAM-3. ICAMs show differences in tissue distribution and inducibility. The recently described ICAM-3 is highly expressed on resting lymphocytes, monocytes and neutrophils. Here, we demonstrate that the whole human epidermal Langerhans cell (LC) population expresses this molecule. Immunohistochemical staining of skin sections with an anti-ICAM-3 monoclonal antibody displayed reactivity with dendritic epidermal cells regularly distributed along the epidermis. Highly-sensitive immunoelectron microscopy procedures, performed on freshly suspended epidermal cells both at transmission and scanning electron microscopic levels, enabled demonstration that the whole LC population expresses cell surface ICAM-3. In contrast, keratinocytes and melanocytes were consistently negative. The prominent expression of ICAM-3 by resident LC could imply a crucial part for this molecule in leucocyte-intercellular interactions in the skin.

AB - Three ligands have been described for the leucocyte integrin LFA-1, namely intercellular adhesion molecule (ICAM)-1; ICAM-2, and ICAM-3. ICAMs show differences in tissue distribution and inducibility. The recently described ICAM-3 is highly expressed on resting lymphocytes, monocytes and neutrophils. Here, we demonstrate that the whole human epidermal Langerhans cell (LC) population expresses this molecule. Immunohistochemical staining of skin sections with an anti-ICAM-3 monoclonal antibody displayed reactivity with dendritic epidermal cells regularly distributed along the epidermis. Highly-sensitive immunoelectron microscopy procedures, performed on freshly suspended epidermal cells both at transmission and scanning electron microscopic levels, enabled demonstration that the whole LC population expresses cell surface ICAM-3. In contrast, keratinocytes and melanocytes were consistently negative. The prominent expression of ICAM-3 by resident LC could imply a crucial part for this molecule in leucocyte-intercellular interactions in the skin.

UR - http://www.scopus.com/inward/record.url?scp=0030048223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030048223&partnerID=8YFLogxK

M3 - Article

C2 - 8745881

AN - SCOPUS:0030048223

VL - 134

SP - 22

EP - 27

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 1

ER -