Human Fanconi A cells are susceptible to TRAIL-induced apoptosis

Simona Pigullo, Elisa Ferretti, Marina Lanciotti, Maurizio Bruschi, Giovanni Candiano, Johanna Svahn, Laura Haneline, Carlo Dufour, Vito Pistoia, Anna Corcione

Research output: Contribution to journalArticlepeer-review

Abstract

Tumour necrosis factor (TNF) contributes to the pathogenesis of bone marrow failure in Fanconi anaemia (FA) patients. The sensitivity of haematopoietic cells from FA, complementation group A (FANCA) subjects, who represent the majority of FA patients, to TNF-related apoptosis-inducing ligand (TRAIL) is unknown. The human lymphoblastoid FANCA HSC072 cell line and the genetically corrected counterpart HSC072FANCA-neo were tested for apoptoptic response to TRAIL using flow cytometry and Western blotting. FANCA cells were more sensitive to TRAIL-induced apoptosis than their corrected counterparts, indicating that TRAIL negatively regulates haematopoietic FANCA cell lines. This effect involved poly(ADP-ribose) polymerase-1 cleavage and caspase-8 activation.

Original languageEnglish
Pages (from-to)315-318
Number of pages4
JournalBritish Journal of Haematology
Volume136
Issue number2
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Apoptosis
  • Caspase-8
  • Fanconi type A cells
  • Poly(ADP-ribose) polymerase-1 cleavage
  • Tumour necrosis factor-related apoptosis-inducing ligand

ASJC Scopus subject areas

  • Hematology

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