Human fetal aorta contains vascular progenitor cells capable of inducing vasculogenesis, angiogenesis, and myogenesis in vitro and in a murine model of peripheral ischemia

Gloria Invernici, Costanza Emanueli, Paolo Madeddu, Silvia Cristini, Sergio Gadau, Anna Benetti, Emilio Ciusani, Giorgio Stassi, Mauro Siragusa, Roberto Nicosia, Cesare Peschle, Umberto Fascio, Augusto Colombo, Tommaso Rizzuti, Eugenio Parati, Giulio Alessandri

Research output: Contribution to journalArticle

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Abstract

Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (ie, angioblasts), is poorly understood. We report the identification of a population of vascular progenitor cells (hVPCs) in the human fetal aorta composed of undifferentiated mesenchymal cells that coexpress endothelial and myogenic markers. Under culture conditions that promoted cell differentiation, hVPCs gave rise to a mixed population of mature endothelial and mural cells when progenitor cells were stimulated with vascular endothelial growth factor-A or platelet-derived growth factor-ββ. hVPCs grew as nonadherent cells and, when embedded in a three-dimensional collagen gel, reorganized into cohesive cellular cords that resembled mature vascular structures. hVPC-conditioned medium contained angiogenic substances (vascular endothelial growth factor-A and angiopoietin-2) and strongly stimulated the proliferation of endothelial cells. We also demonstrate the therapeutic efficacy of a small number of hVPCs transplanted into ischemic limb muscle of immunodeficient mice. hVPCs markedly improved neovascularization and inhibited the loss of endogenous endothelial cells and myocytes, thus ameliorating the clinical outcome from ischemia. We conclude that fetal aorta represents an important source for the investigation of the phenotypic and functional features of human vascular progenitor cells.

Original languageEnglish
Pages (from-to)1879-1892
Number of pages14
JournalAmerican Journal of Pathology
Volume170
Issue number6
DOIs
Publication statusPublished - Jun 2007

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Muscle Development
Blood Vessels
Aorta
Stem Cells
Ischemia
Endothelial Cells
Vascular Endothelial Growth Factor A
Angiopoietin-2
Platelet-Derived Growth Factor
Conditioned Culture Medium
Muscle Cells
Population
Cell Differentiation
Fetus
Collagen
Extremities
Gels
In Vitro Techniques
Muscles

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Human fetal aorta contains vascular progenitor cells capable of inducing vasculogenesis, angiogenesis, and myogenesis in vitro and in a murine model of peripheral ischemia. / Invernici, Gloria; Emanueli, Costanza; Madeddu, Paolo; Cristini, Silvia; Gadau, Sergio; Benetti, Anna; Ciusani, Emilio; Stassi, Giorgio; Siragusa, Mauro; Nicosia, Roberto; Peschle, Cesare; Fascio, Umberto; Colombo, Augusto; Rizzuti, Tommaso; Parati, Eugenio; Alessandri, Giulio.

In: American Journal of Pathology, Vol. 170, No. 6, 06.2007, p. 1879-1892.

Research output: Contribution to journalArticle

Invernici, Gloria ; Emanueli, Costanza ; Madeddu, Paolo ; Cristini, Silvia ; Gadau, Sergio ; Benetti, Anna ; Ciusani, Emilio ; Stassi, Giorgio ; Siragusa, Mauro ; Nicosia, Roberto ; Peschle, Cesare ; Fascio, Umberto ; Colombo, Augusto ; Rizzuti, Tommaso ; Parati, Eugenio ; Alessandri, Giulio. / Human fetal aorta contains vascular progenitor cells capable of inducing vasculogenesis, angiogenesis, and myogenesis in vitro and in a murine model of peripheral ischemia. In: American Journal of Pathology. 2007 ; Vol. 170, No. 6. pp. 1879-1892.
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AU - Cristini, Silvia

AU - Gadau, Sergio

AU - Benetti, Anna

AU - Ciusani, Emilio

AU - Stassi, Giorgio

AU - Siragusa, Mauro

AU - Nicosia, Roberto

AU - Peschle, Cesare

AU - Fascio, Umberto

AU - Colombo, Augusto

AU - Rizzuti, Tommaso

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AU - Alessandri, Giulio

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