TY - JOUR
T1 - Human gamma interferon enhances release from phytohemagglutinin-stimulated T4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells
AU - Piacibello, W.
AU - Lu, L.
AU - Williams, D.
AU - Aglietta, M.
AU - Rubin, B. Y.
AU - Cooper, S.
AU - Wachter, M.
AU - Gavosto, F.
AU - Broxmeyer, H. E.
PY - 1986
Y1 - 1986
N2 - Human gamma interferon (HuIFNγ) was evaluated for its effects on the release from human peripheral blood T lymphocytes (>98% pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granolocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFNγ. In the presence of PHA, pure natural HuIFNγ at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1%, 0.5%, and 1% PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1% and 0.5% PHA. The enhanced release of colony-stimulating activities was not due to HuIFNγ-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFNγ were neutralized with a monoclonal anti-natural HuIFNγ, and recombinant HuIFNγ mimicked the enhancing effects of the natural HuIFNγ. This enhancing effect was noted only when HuIFNγ was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (>98% pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFNγ was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFNγ is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFNγ and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.
AB - Human gamma interferon (HuIFNγ) was evaluated for its effects on the release from human peripheral blood T lymphocytes (>98% pure) stimulated by phytohemagglutinin (PHA) of activities that can stimulate granolocyte-macrophage (CFU-GM) colonies and clusters, erythroid (BFU-E) bursts, and mixed (CFU-GEMM) colonies. T lymphocytes did not release these activities in the absence of PHA with or without HuIFNγ. In the presence of PHA, pure natural HuIFNγ at concentrations of 0.1 to 100 U/mL significantly enhanced release of these colony-stimulating activities. Although enhanced release of granulocyte-macrophage colony-stimulating activities were noted when T lymphocytes were added to the conditioning medium in the presence of 0.1%, 0.5%, and 1% PHA, enhanced release of burst-promoting and mixed colony activities was seen only in the presence of 0.1% and 0.5% PHA. The enhanced release of colony-stimulating activities was not due to HuIFNγ-suppression of the release from PHA-stimulated T lymphocytes of suppressor molecules. The enhancing effects of natural HuIFNγ were neutralized with a monoclonal anti-natural HuIFNγ, and recombinant HuIFNγ mimicked the enhancing effects of the natural HuIFNγ. This enhancing effect was noted only when HuIFNγ was added with the T lymphocytes and PHA during the first 24 hours of incubation. T lymphocytes were separated into T4+, T8-, T8+, and T4- subsets (>98% pure for the appropriate phenotypes) after incubation with OKT4- and OKT8- monoclonal antibodies and sorting on a fluorescence-activated cell sorter (FACS). All types of colony-stimulating activities were released from each population after stimulation with PHA, but enhanced release of these activities in the presence of HuIFNγ was only detected with the T4+ or T8- subsets of lymphocytes. It cannot be concluded from these studies whether HuIFNγ is enhancing the release of one or several types of colony-stimulating activities, but these studies suggest a role for HuIFNγ and T4+ lymphocyte subsets in the regulation in vitro of the release of colony-stimulating activities.
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M3 - Article
C2 - 3096401
AN - SCOPUS:0022863640
VL - 68
SP - 1339
EP - 1347
JO - Blood
JF - Blood
SN - 0006-4971
IS - 6
ER -