TY - JOUR
T1 - Human glioma tumors express high levels of the chemokine receptor CX3CR1
AU - Locatelli, Marco
AU - Boiocchi, Leonardo
AU - Ferrero, Stefano
AU - Boneschi, Filippo Martinelli
AU - Zavanone, Mario
AU - Pesce, Samantha
AU - Allavena, Paola
AU - Gaini, Sergio Maria
AU - Bello, Lorenzo
AU - Mantovani, Alberto
PY - 2010/3
Y1 - 2010/3
N2 - The chemokine receptor CX3CR1 and its cognate ligand CX3CL1 (also known as fractalkine), are involved in central nervous system pathophysiology, in particular, in the cross-talk between neurons and microglia. It was therefore important to investigate the expression of CX3CR1 in gliomas, the most frequently occurring, malignant brain tumors. In a consecutive series of 70 patients with primary, central nervous glial tumors, CX3CR1 was highly expressed in tumor cells as assessed by RT-PCR mRNA and protein levels, and by immunohistochemistry, while the corresponding normal cells were negative. Receptor immuno-positivity did not correlate with histology, grade, chromosomal (1p,19q) deletion, or with methylation of the DNA repair gene promoter MGMT (O6-methylguanine-DNA methyltransferase). Thus, CX3CR1 expression is a frequent event in gliomas, irrespective of tumor classification and clinical severity. The molecular basis underlying CX3CR1 up-regulation and its functional biological significance remain to be determined.
AB - The chemokine receptor CX3CR1 and its cognate ligand CX3CL1 (also known as fractalkine), are involved in central nervous system pathophysiology, in particular, in the cross-talk between neurons and microglia. It was therefore important to investigate the expression of CX3CR1 in gliomas, the most frequently occurring, malignant brain tumors. In a consecutive series of 70 patients with primary, central nervous glial tumors, CX3CR1 was highly expressed in tumor cells as assessed by RT-PCR mRNA and protein levels, and by immunohistochemistry, while the corresponding normal cells were negative. Receptor immuno-positivity did not correlate with histology, grade, chromosomal (1p,19q) deletion, or with methylation of the DNA repair gene promoter MGMT (O6-methylguanine-DNA methyltransferase). Thus, CX3CR1 expression is a frequent event in gliomas, irrespective of tumor classification and clinical severity. The molecular basis underlying CX3CR1 up-regulation and its functional biological significance remain to be determined.
KW - Chemokines
KW - CX3CL1
KW - CX3CR1
KW - Gliomas
KW - Histochemistry
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U2 - 10.1684/ecn.2009.0184
DO - 10.1684/ecn.2009.0184
M3 - Article
C2 - 20146987
AN - SCOPUS:77149179418
VL - 21
SP - 27
EP - 33
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
SN - 0893-6692
IS - 1
ER -