Abstract
The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-6 on clonogenic growth of blast-cell progenitors from 19 immunologically defined CD10-positive B-lineage acute lymphoblastic leukemias (ALL) coexpressing (My+ALLs) or not (My-ALLs) myeloid antigens have been studied. Our results demonstrate that GM-CSF was able to support the clonogenic growth of blast cells from My+ALLs, being totally ineffective on My-All samples. Accordingly, both α and β chains of GM-CSF receptor (R) were expressed by My+ALL blasts, as investigated by reverse-transcriptase polymerase chain reaction (RT-PCR). Colony cells from GM-CSF-stimulated My+ALL cultures displayed the same immunophenotype as primary leukemic cells at diagnosis (CD10+, CD19+, CD22+), and retained the expression of myeloid-associated antigens and of GM-CSF-R transcripts. Moreover, My+ALL blasts showed a preferential sensitivity to the growth-promoting activity of IL-3 and IL-6, as compared with My-ALL cells. In addition to rearrangements of the JH region of immunoglobulin genes, My+ALL cells showed aberrant rearrangements of γ (three cases) and β (two cases) T-cell receptor genes, as well as of bcr sequences (three cases). Our data, showing an unexpected cross-lineage response of My+ALLs to GM-CSF, and their preferential stimulation by IL-3 and IL-6, as compared with My-ALLs, further support the concept that My+ALLs represent a separate entity with unique biological features.
| Original language | English |
|---|---|
| Pages (from-to) | 141-151 |
| Number of pages | 11 |
| Journal | Cytokines, Cellular and Molecular Therapy |
| Volume | 3 |
| Issue number | 3 |
| Publication status | Published - Sep 1997 |
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Keywords
- B-lineage ALL
- Biphenotypic leukemia
- Colony assay
- GM-CSF
- Hemopoietic growth factors
ASJC Scopus subject areas
- Pharmacology
- Immunology and Allergy
- Immunology
Cite this
Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens. / Gattei, Valter; Aldinucci, Donatella; Attadia, Vincenza; Degan, Massimo; Alosi, Maria Stella; De Iuliis, Angela; Babare, Roberta; Rossi, Francesca Maria; Rupolo, Maurizio; Zagonel, Vittorina; Pinto, Antonio.
In: Cytokines, Cellular and Molecular Therapy, Vol. 3, No. 3, 09.1997, p. 141-151.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens
AU - Gattei, Valter
AU - Aldinucci, Donatella
AU - Attadia, Vincenza
AU - Degan, Massimo
AU - Alosi, Maria Stella
AU - De Iuliis, Angela
AU - Babare, Roberta
AU - Rossi, Francesca Maria
AU - Rupolo, Maurizio
AU - Zagonel, Vittorina
AU - Pinto, Antonio
PY - 1997/9
Y1 - 1997/9
N2 - The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-6 on clonogenic growth of blast-cell progenitors from 19 immunologically defined CD10-positive B-lineage acute lymphoblastic leukemias (ALL) coexpressing (My+ALLs) or not (My-ALLs) myeloid antigens have been studied. Our results demonstrate that GM-CSF was able to support the clonogenic growth of blast cells from My+ALLs, being totally ineffective on My-All samples. Accordingly, both α and β chains of GM-CSF receptor (R) were expressed by My+ALL blasts, as investigated by reverse-transcriptase polymerase chain reaction (RT-PCR). Colony cells from GM-CSF-stimulated My+ALL cultures displayed the same immunophenotype as primary leukemic cells at diagnosis (CD10+, CD19+, CD22+), and retained the expression of myeloid-associated antigens and of GM-CSF-R transcripts. Moreover, My+ALL blasts showed a preferential sensitivity to the growth-promoting activity of IL-3 and IL-6, as compared with My-ALL cells. In addition to rearrangements of the JH region of immunoglobulin genes, My+ALL cells showed aberrant rearrangements of γ (three cases) and β (two cases) T-cell receptor genes, as well as of bcr sequences (three cases). Our data, showing an unexpected cross-lineage response of My+ALLs to GM-CSF, and their preferential stimulation by IL-3 and IL-6, as compared with My-ALLs, further support the concept that My+ALLs represent a separate entity with unique biological features.
AB - The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-6 on clonogenic growth of blast-cell progenitors from 19 immunologically defined CD10-positive B-lineage acute lymphoblastic leukemias (ALL) coexpressing (My+ALLs) or not (My-ALLs) myeloid antigens have been studied. Our results demonstrate that GM-CSF was able to support the clonogenic growth of blast cells from My+ALLs, being totally ineffective on My-All samples. Accordingly, both α and β chains of GM-CSF receptor (R) were expressed by My+ALL blasts, as investigated by reverse-transcriptase polymerase chain reaction (RT-PCR). Colony cells from GM-CSF-stimulated My+ALL cultures displayed the same immunophenotype as primary leukemic cells at diagnosis (CD10+, CD19+, CD22+), and retained the expression of myeloid-associated antigens and of GM-CSF-R transcripts. Moreover, My+ALL blasts showed a preferential sensitivity to the growth-promoting activity of IL-3 and IL-6, as compared with My-ALL cells. In addition to rearrangements of the JH region of immunoglobulin genes, My+ALL cells showed aberrant rearrangements of γ (three cases) and β (two cases) T-cell receptor genes, as well as of bcr sequences (three cases). Our data, showing an unexpected cross-lineage response of My+ALLs to GM-CSF, and their preferential stimulation by IL-3 and IL-6, as compared with My-ALLs, further support the concept that My+ALLs represent a separate entity with unique biological features.
KW - B-lineage ALL
KW - Biphenotypic leukemia
KW - Colony assay
KW - GM-CSF
KW - Hemopoietic growth factors
UR - http://www.scopus.com/inward/record.url?scp=9844253881&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9844253881&partnerID=8YFLogxK
M3 - Article
VL - 3
SP - 141
EP - 151
JO - Cytokines, Cellular and Molecular Therapy
JF - Cytokines, Cellular and Molecular Therapy
SN - 1368-4736
IS - 3
ER -