Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens

Valter Gattei, Donatella Aldinucci, Vincenza Attadia, Massimo Degan, Maria Stella Alosi, Angela De Iuliis, Roberta Babare, Francesca Maria Rossi, Maurizio Rupolo, Vittorina Zagonel, Antonio Pinto

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-6 on clonogenic growth of blast-cell progenitors from 19 immunologically defined CD10-positive B-lineage acute lymphoblastic leukemias (ALL) coexpressing (My+ALLs) or not (My-ALLs) myeloid antigens have been studied. Our results demonstrate that GM-CSF was able to support the clonogenic growth of blast cells from My+ALLs, being totally ineffective on My-All samples. Accordingly, both α and β chains of GM-CSF receptor (R) were expressed by My+ALL blasts, as investigated by reverse-transcriptase polymerase chain reaction (RT-PCR). Colony cells from GM-CSF-stimulated My+ALL cultures displayed the same immunophenotype as primary leukemic cells at diagnosis (CD10+, CD19+, CD22+), and retained the expression of myeloid-associated antigens and of GM-CSF-R transcripts. Moreover, My+ALL blasts showed a preferential sensitivity to the growth-promoting activity of IL-3 and IL-6, as compared with My-ALL cells. In addition to rearrangements of the JH region of immunoglobulin genes, My+ALL cells showed aberrant rearrangements of γ (three cases) and β (two cases) T-cell receptor genes, as well as of bcr sequences (three cases). Our data, showing an unexpected cross-lineage response of My+ALLs to GM-CSF, and their preferential stimulation by IL-3 and IL-6, as compared with My-ALLs, further support the concept that My+ALLs represent a separate entity with unique biological features.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalCytokines, Cellular and Molecular Therapy
Volume3
Issue number3
Publication statusPublished - Sep 1997

Keywords

  • B-lineage ALL
  • Biphenotypic leukemia
  • Colony assay
  • GM-CSF
  • Hemopoietic growth factors

ASJC Scopus subject areas

  • Pharmacology
  • Immunology and Allergy
  • Immunology

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