Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens

Valter Gattei, Donatella Aldinucci, Vincenza Attadia, Massimo Degan, Maria Stella Alosi, Angela De Iuliis, Roberta Babare, Francesca Maria Rossi, Maurizio Rupolo, Vittorina Zagonel, Antonio Pinto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-6 on clonogenic growth of blast-cell progenitors from 19 immunologically defined CD10-positive B-lineage acute lymphoblastic leukemias (ALL) coexpressing (My+ALLs) or not (My-ALLs) myeloid antigens have been studied. Our results demonstrate that GM-CSF was able to support the clonogenic growth of blast cells from My+ALLs, being totally ineffective on My-All samples. Accordingly, both α and β chains of GM-CSF receptor (R) were expressed by My+ALL blasts, as investigated by reverse-transcriptase polymerase chain reaction (RT-PCR). Colony cells from GM-CSF-stimulated My+ALL cultures displayed the same immunophenotype as primary leukemic cells at diagnosis (CD10+, CD19+, CD22+), and retained the expression of myeloid-associated antigens and of GM-CSF-R transcripts. Moreover, My+ALL blasts showed a preferential sensitivity to the growth-promoting activity of IL-3 and IL-6, as compared with My-ALL cells. In addition to rearrangements of the JH region of immunoglobulin genes, My+ALL cells showed aberrant rearrangements of γ (three cases) and β (two cases) T-cell receptor genes, as well as of bcr sequences (three cases). Our data, showing an unexpected cross-lineage response of My+ALLs to GM-CSF, and their preferential stimulation by IL-3 and IL-6, as compared with My-ALLs, further support the concept that My+ALLs represent a separate entity with unique biological features.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalCytokines, Cellular and Molecular Therapy
Volume3
Issue number3
Publication statusPublished - Sep 1997

Fingerprint

Granulocyte-Macrophage Colony-Stimulating Factor
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Antigens
Interleukin-3
Growth
Interleukin-6
Granulocyte-Macrophage Colony-Stimulating Factor Receptors
T-Cell Receptor Genes
Immunoglobulin Genes
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells

Keywords

  • B-lineage ALL
  • Biphenotypic leukemia
  • Colony assay
  • GM-CSF
  • Hemopoietic growth factors

ASJC Scopus subject areas

  • Pharmacology
  • Immunology and Allergy
  • Immunology

Cite this

Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens. / Gattei, Valter; Aldinucci, Donatella; Attadia, Vincenza; Degan, Massimo; Alosi, Maria Stella; De Iuliis, Angela; Babare, Roberta; Rossi, Francesca Maria; Rupolo, Maurizio; Zagonel, Vittorina; Pinto, Antonio.

In: Cytokines, Cellular and Molecular Therapy, Vol. 3, No. 3, 09.1997, p. 141-151.

Research output: Contribution to journalArticle

Gattei, Valter ; Aldinucci, Donatella ; Attadia, Vincenza ; Degan, Massimo ; Alosi, Maria Stella ; De Iuliis, Angela ; Babare, Roberta ; Rossi, Francesca Maria ; Rupolo, Maurizio ; Zagonel, Vittorina ; Pinto, Antonio. / Human granulocyte-macrophage colony-stimulating factor supports the clonogenic growth of B-lineage acute lymphoblastic leukemias expressing myeloid antigens. In: Cytokines, Cellular and Molecular Therapy. 1997 ; Vol. 3, No. 3. pp. 141-151.
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AU - Attadia, Vincenza

AU - Degan, Massimo

AU - Alosi, Maria Stella

AU - De Iuliis, Angela

AU - Babare, Roberta

AU - Rossi, Francesca Maria

AU - Rupolo, Maurizio

AU - Zagonel, Vittorina

AU - Pinto, Antonio

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