TY - JOUR
T1 - Human Haemato-Endothelial Precursors
T2 - Cord Blood CD34+ Cells Produce Haemogenic Endothelium
AU - Pelosi, Elvira
AU - Castelli, Germana
AU - Martin-Padura, Ines
AU - Bordoni, Veronica
AU - Santoro, Simona
AU - Conigliaro, Alice
AU - Cerio, Anna Maria
AU - de Santis Puzzonia, Marco
AU - Marighetti, Paola
AU - Biffoni, Mauro
AU - Alonzi, Tonino
AU - Amicone, Laura
AU - Alcalay, Myriam
AU - Bertolini, Francesco
AU - Testa, Ugo
AU - Tripodi, Marco
PY - 2012/12/4
Y1 - 2012/12/4
N2 - Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level.
AB - Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give rise to functional vasculature in vivo, if further instructed by haematopoietic growth factors, first switch to transitional CD144+45+ cells and then to haematopoietic cells. These results highlight the plasticity of haemato-endhothelial precursors in human post-natal life. Furthermore, these studies may provide highly enriched populations of human post-fetal haemogenic endothelium, paving the way for innovative projects at a basic and possibly clinical level.
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U2 - 10.1371/journal.pone.0051109
DO - 10.1371/journal.pone.0051109
M3 - Article
C2 - 23226561
AN - SCOPUS:84870729547
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 12
M1 - e51109
ER -