Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels

Paola Secchiero, Davide Gibellini, Louis Flamand, Iole Robuffo, Marco Marchisio, Silvano Capitani, Robert C. Gallo, Giorgio Zauli

Research output: Contribution to journalArticle

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Abstract

In this study, we investigated the fate of the CD4 Ag during infection of CD4+ T cells with the T lymphotrophic human herpesvirus 7 (HHV-7), using the SupT1 lymphoblastoid T cell line as a model system. The following points were established: 1) productive infection with HHV-7 was required to obtain persistent down-modulation of surface CD4 (CD4SURF); 2) at 6 to 9 days postinfection, when approximately 50 to 60% of SupT1 cells still showed a CD4SURF dim positivity, a drastic loss of total CD4 protein was found by either Western blot or immunoprecipitation experiments; 3) a block in CD4 protein production was demonstrated by a radioimmunoprecipitation assay; 4) analysis of the mRNA steady-state levels and transfection studies performed with a plasmid containing the CD4 promoter/enhancer regions in front of the luciferase gene indicated that HHV-7 infection has a suppressive effect on CD4 transcription; 5) both CD4SURF and intracellular CD4 (CD4INTRA) were reduced in HHV-7-infected cells with respect to uninfected controls, but the loss of CD4SURF was more dramatic than that of CD4INTRA; 6) immunogold labeling and electron microscopy demonstrated that CD4INTRA co-localized with HHV-7 Ags within the same subcellular compartments of infected cells; and 7) the total amount of the tyrosine kinase p56lck and tyrosine phosphorylated p56lck levels were unchanged in HHV-7-infected versus uninfected cells.

Original languageEnglish
Pages (from-to)3412-3423
Number of pages12
JournalJournal of Immunology
Volume159
Issue number7
Publication statusPublished - Oct 1 1997

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Human Herpesvirus 7
CD4 Antigens
Down-Regulation
Lymphocytes
T-Lymphocytes
Radioimmunoprecipitation Assay
Herpesviridae Infections
Infection
Luciferases
Immunoprecipitation
Genetic Promoter Regions
Protein-Tyrosine Kinases
Transfection
Tyrosine
Electron Microscopy
Proteins
Plasmids
Western Blotting
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Secchiero, P., Gibellini, D., Flamand, L., Robuffo, I., Marchisio, M., Capitani, S., ... Zauli, G. (1997). Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels. Journal of Immunology, 159(7), 3412-3423.

Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels. / Secchiero, Paola; Gibellini, Davide; Flamand, Louis; Robuffo, Iole; Marchisio, Marco; Capitani, Silvano; Gallo, Robert C.; Zauli, Giorgio.

In: Journal of Immunology, Vol. 159, No. 7, 01.10.1997, p. 3412-3423.

Research output: Contribution to journalArticle

Secchiero, P, Gibellini, D, Flamand, L, Robuffo, I, Marchisio, M, Capitani, S, Gallo, RC & Zauli, G 1997, 'Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels', Journal of Immunology, vol. 159, no. 7, pp. 3412-3423.
Secchiero P, Gibellini D, Flamand L, Robuffo I, Marchisio M, Capitani S et al. Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels. Journal of Immunology. 1997 Oct 1;159(7):3412-3423.
Secchiero, Paola ; Gibellini, Davide ; Flamand, Louis ; Robuffo, Iole ; Marchisio, Marco ; Capitani, Silvano ; Gallo, Robert C. ; Zauli, Giorgio. / Human Herpesvirus 7 Induces the Down-Regulation of CD4 Antigen in Lymphoid T Cells Without Affecting p56lck Levels. In: Journal of Immunology. 1997 ; Vol. 159, No. 7. pp. 3412-3423.
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