Human herpesvirus 7 infection induces profound cell cycle perturbations coupled to disregulation of cdc2 and cyclin B and polyploidization of CD4+ T cells

Paola Secchiero, Lucia Bertolaso, Luca Casareto, Davide Gibellini, Marco Vitale, Kristi Bemis, Arrigo Aleotti, Silvano Capitani, Genoveffa Franchini, Robert C. Gallo, Giorgio Zauli

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29 Citations (Scopus)

Abstract

Human herpesvirus 7 (HHV-7) infection of both primary CD4+ T lymphocytes and SupT1 lymphoblastoid T-cell line induced a progressive accumulation of cells exibiting a gap 2/mitosis (G2/M) and polyploid content coupled to an increased cell size. The expression of both cyclin-dependent kinase cdc2 and cyclin B was increased in HHV-7-infected cells with respect to the uninfected ones. Moreover, the simultaneous flow cytometric analysis of cyclin B and DNA content showed that cyclin B expression was not only increased but also unscheduled with respect to its usual cell cycle pattern. However, the levels of kinase activity associated to cdc2 were decreased in HHV-7-infected cells with respect to uninfected cultures. To elucidate the origin of the enlarged HHV-7-infected cells, extensive electron and confocal microscopy analyses were performed. Membrane fusion events associated to cytoplasmic bridges, which characterize the formation of syncytia, were never observed. On the other hand, analysis of serial sections of the same cells strongly suggested that enlarged HHV-7-infected cells contained a single polylobated nucleus. This was confirmed by flow cytometry analysis performed on nuclei isolated from HHV-7-infected cells, which showed multiple peaks with a DNA content >4n. Taken together, these data indicate that giant cells, which represent the hallmark of in vitro HHV-7 infection, arise from single CD4+ T cells undergoing a process of polyploidization.

Original languageEnglish
Pages (from-to)1685-1696
Number of pages12
JournalBlood
Volume92
Issue number5
Publication statusPublished - Sep 1 1998

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Human Herpesvirus 7
Cyclin B
Herpesviridae Infections
T-cells
Cell Cycle
Cells
T-Lymphocytes
Cyclin-Dependent Kinases
Flow cytometry
Confocal microscopy
DNA
Electron microscopy
Giant Cells
Phosphotransferases
Fusion reactions
Membranes
Membrane Fusion
Polyploidy
Cell Size
Mitosis

ASJC Scopus subject areas

  • Hematology

Cite this

Secchiero, P., Bertolaso, L., Casareto, L., Gibellini, D., Vitale, M., Bemis, K., ... Zauli, G. (1998). Human herpesvirus 7 infection induces profound cell cycle perturbations coupled to disregulation of cdc2 and cyclin B and polyploidization of CD4+ T cells. Blood, 92(5), 1685-1696.

Human herpesvirus 7 infection induces profound cell cycle perturbations coupled to disregulation of cdc2 and cyclin B and polyploidization of CD4+ T cells. / Secchiero, Paola; Bertolaso, Lucia; Casareto, Luca; Gibellini, Davide; Vitale, Marco; Bemis, Kristi; Aleotti, Arrigo; Capitani, Silvano; Franchini, Genoveffa; Gallo, Robert C.; Zauli, Giorgio.

In: Blood, Vol. 92, No. 5, 01.09.1998, p. 1685-1696.

Research output: Contribution to journalArticle

Secchiero, P, Bertolaso, L, Casareto, L, Gibellini, D, Vitale, M, Bemis, K, Aleotti, A, Capitani, S, Franchini, G, Gallo, RC & Zauli, G 1998, 'Human herpesvirus 7 infection induces profound cell cycle perturbations coupled to disregulation of cdc2 and cyclin B and polyploidization of CD4+ T cells', Blood, vol. 92, no. 5, pp. 1685-1696.
Secchiero P, Bertolaso L, Casareto L, Gibellini D, Vitale M, Bemis K et al. Human herpesvirus 7 infection induces profound cell cycle perturbations coupled to disregulation of cdc2 and cyclin B and polyploidization of CD4+ T cells. Blood. 1998 Sep 1;92(5):1685-1696.
Secchiero, Paola ; Bertolaso, Lucia ; Casareto, Luca ; Gibellini, Davide ; Vitale, Marco ; Bemis, Kristi ; Aleotti, Arrigo ; Capitani, Silvano ; Franchini, Genoveffa ; Gallo, Robert C. ; Zauli, Giorgio. / Human herpesvirus 7 infection induces profound cell cycle perturbations coupled to disregulation of cdc2 and cyclin B and polyploidization of CD4+ T cells. In: Blood. 1998 ; Vol. 92, No. 5. pp. 1685-1696.
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abstract = "Human herpesvirus 7 (HHV-7) infection of both primary CD4+ T lymphocytes and SupT1 lymphoblastoid T-cell line induced a progressive accumulation of cells exibiting a gap 2/mitosis (G2/M) and polyploid content coupled to an increased cell size. The expression of both cyclin-dependent kinase cdc2 and cyclin B was increased in HHV-7-infected cells with respect to the uninfected ones. Moreover, the simultaneous flow cytometric analysis of cyclin B and DNA content showed that cyclin B expression was not only increased but also unscheduled with respect to its usual cell cycle pattern. However, the levels of kinase activity associated to cdc2 were decreased in HHV-7-infected cells with respect to uninfected cultures. To elucidate the origin of the enlarged HHV-7-infected cells, extensive electron and confocal microscopy analyses were performed. Membrane fusion events associated to cytoplasmic bridges, which characterize the formation of syncytia, were never observed. On the other hand, analysis of serial sections of the same cells strongly suggested that enlarged HHV-7-infected cells contained a single polylobated nucleus. This was confirmed by flow cytometry analysis performed on nuclei isolated from HHV-7-infected cells, which showed multiple peaks with a DNA content >4n. Taken together, these data indicate that giant cells, which represent the hallmark of in vitro HHV-7 infection, arise from single CD4+ T cells undergoing a process of polyploidization.",
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