Human herpesvirus 8 DNA quantification in matched plasma and PBMCs samples of patients with HHV8-related lymphoproliferative diseases

Rosamaria Tedeschi, Alessia Marus, Ettore Bidoli, Cecilia Simonelli, Paolo De Paoli

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: The quantitative evaluation of human herpesvirus 8 (HHV8) DNA is not well described in the clinical management of HHV8-related lymphoproliferative diseases. Objectives: To evaluate and to compare HHV8 viral load in different blood compartments from patients with multicentric Castleman's disease (MCD), primary effusion lymphoma (PEL) and HHV8-associated solid lymphoma (SLY) and to establish which clinical sample would be preferable for HHV8 DNA testing. Study design: We assessed HHV8 DNA in plasma and peripheral blood mononuclear cells (PBMCs) paired samples from 7 PEL, 8 MCD, 2 SLY HIV+ patients at the diagnosis and during the course of the illness by using a real time PCR assay. Results: HHV8 viremia was always detectable at diagnosis. HHV8 DNA levels were correlated in matched pairs of samples at diagnosis and during follow-up (Spearman correlation coefficient: r = 0.83, p <0.001 and r = 0.73, p <0.001, respectively). The performance characteristics of the PCR assay with both materials did not show disparity by the analysis of the receiver operating characteristic (ROC) curve (X1 2 = 0.50; p = 0.48). Conclusions: Plasma or PBMCs are both adequate samples for HHV8 DNA quantification and Real time PCR provides a reliable method to estimate viral replication in patients with HHV8-related lymphoproliferations, where HHV8 viral load is a consistent feature.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalJournal of Clinical Virology
Volume43
Issue number3
DOIs
Publication statusPublished - Nov 2008

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Human Herpesvirus 8
Blood Cells
DNA
Primary Effusion Lymphoma
Viral Load
Real-Time Polymerase Chain Reaction
Lymphoma
Viremia
ROC Curve
HIV

Keywords

  • HHV8
  • MCD
  • PBMCs
  • PEL
  • Plasma
  • Viral load

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Human herpesvirus 8 DNA quantification in matched plasma and PBMCs samples of patients with HHV8-related lymphoproliferative diseases. / Tedeschi, Rosamaria; Marus, Alessia; Bidoli, Ettore; Simonelli, Cecilia; De Paoli, Paolo.

In: Journal of Clinical Virology, Vol. 43, No. 3, 11.2008, p. 255-259.

Research output: Contribution to journalArticle

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abstract = "Background: The quantitative evaluation of human herpesvirus 8 (HHV8) DNA is not well described in the clinical management of HHV8-related lymphoproliferative diseases. Objectives: To evaluate and to compare HHV8 viral load in different blood compartments from patients with multicentric Castleman's disease (MCD), primary effusion lymphoma (PEL) and HHV8-associated solid lymphoma (SLY) and to establish which clinical sample would be preferable for HHV8 DNA testing. Study design: We assessed HHV8 DNA in plasma and peripheral blood mononuclear cells (PBMCs) paired samples from 7 PEL, 8 MCD, 2 SLY HIV+ patients at the diagnosis and during the course of the illness by using a real time PCR assay. Results: HHV8 viremia was always detectable at diagnosis. HHV8 DNA levels were correlated in matched pairs of samples at diagnosis and during follow-up (Spearman correlation coefficient: r = 0.83, p <0.001 and r = 0.73, p <0.001, respectively). The performance characteristics of the PCR assay with both materials did not show disparity by the analysis of the receiver operating characteristic (ROC) curve (X1 2 = 0.50; p = 0.48). Conclusions: Plasma or PBMCs are both adequate samples for HHV8 DNA quantification and Real time PCR provides a reliable method to estimate viral replication in patients with HHV8-related lymphoproliferations, where HHV8 viral load is a consistent feature.",
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