Human herpesvirus 8 infection in patients with cutaneous lymphoproliferative diseases

Elisabetta Trento, Concetta Castilletti, Carmela Ferraro, Ilaria Lesnoni La Parola, Anna Mussi, Luca Muscardin, Valentina Bordignon, Giovanna D'Agosto, Ada Amantea, Antonio Mastroianni, Franco Ameglio, Joachim Fluhr, Paola Cordiali-Fei

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Abstract

Objective: To investigate the prevalence of human herpesvirus 8 (HHV-8; Kaposi sarcoma-associated herpes-virus) infection in patients with lymphoproliferative skin diseases such as large-plaque parapsoriasis (LPP) and mycosis fungoides compared with inflammatory cutaneous conditions or healthy control subjects. Design: A survey study was undertaken in 123 subjects with various clinical conditions. Setting: All patients had been seen in the Dermatology Department of the San Gallicano Dermatology Institute, Rome, Italy, in the last 2 years. Patients: Forty-five patients with inflammatory or autoimmune cutaneous diseases, 50 healthy control subjects, 10 patients with LPP, 12 patients with mycosis fungoides, and 6 patients with classic Kaposi sarcoma were included in the study. Main Outcome Measures: The prevalence of HHV-8 infection was investigated with serologic studies using the gold standard assay based on body cavity-based B-cell lymphoma-1 cells latently infected with HHV-8. The presence of HHV-8 conserved sequence, corresponding to open reading frame 26, was also assessed in the peripheral blood and lesion tissue samples from patients with lymphoproliferative cutaneous diseases with nested polymerase chain reaction. The presence and distribution of cell types infected with HHV-8 in the lesion tissues was determined with immunohistochemical staining with the monoclonal antibody directed against the latent nuclear antigen-1 of HHV-8 encoded by open reading frame 73. Results: In healthy control subjects and patients with inflammatory skin diseases, 13.9% were found to have antibody against HHV-8, consistent with the seroprevalence population in Italy. A highly significant association of HHV-8 infection and LPP was found (100%) compared with mycosis fungoides (25%). The peripheral blood mononuclear cells in 8 of 10 patients with LPP were found to harbor viral sequences at nested polymerase chain reaction, whereas none of them had a detectable serum viral load. All LPP lesion tissue samples were positive for HHV-8-encoded open reading frame 26, and the presence of HHV-8-infected cells was confirmed by immunohistochemistry profiles performed on paraffin-embedded tissues from 4 of 10 patients. The positive cell types included endothelial cells and the infiltrating dermal lymphocytes, characteristic hallmarks of LPP. Analysis of T-cell receptor γ chain rearrangements in lesion tissue from our patients confirmed the lack of a significant association between T-cell clonality and LPP. Conclusion: These data suggest that HHV-8 may play a role in the onset of LPP, a disease whose cause and evolution are still undefined and which has often been considered the early stage of mycosis fungoides.

Original languageEnglish
Pages (from-to)1235-1242
Number of pages8
JournalArchives of Dermatology
Volume141
Issue number10
DOIs
Publication statusPublished - Oct 2005

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Herpesviridae Infections
Human Herpesvirus 8
Parapsoriasis
Skin Diseases
Mycosis Fungoides
Open Reading Frames
Healthy Volunteers
Kaposi's Sarcoma
Dermatology
Italy
Satellite Viruses
Polymerase Chain Reaction
Skin
Conserved Sequence
Seroepidemiologic Studies
B-Cell Lymphoma
Virus Diseases
T-Cell Antigen Receptor
Infection
Viral Load

ASJC Scopus subject areas

  • Dermatology

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Human herpesvirus 8 infection in patients with cutaneous lymphoproliferative diseases. / Trento, Elisabetta; Castilletti, Concetta; Ferraro, Carmela; Lesnoni La Parola, Ilaria; Mussi, Anna; Muscardin, Luca; Bordignon, Valentina; D'Agosto, Giovanna; Amantea, Ada; Mastroianni, Antonio; Ameglio, Franco; Fluhr, Joachim; Cordiali-Fei, Paola.

In: Archives of Dermatology, Vol. 141, No. 10, 10.2005, p. 1235-1242.

Research output: Contribution to journalArticle

Trento, Elisabetta ; Castilletti, Concetta ; Ferraro, Carmela ; Lesnoni La Parola, Ilaria ; Mussi, Anna ; Muscardin, Luca ; Bordignon, Valentina ; D'Agosto, Giovanna ; Amantea, Ada ; Mastroianni, Antonio ; Ameglio, Franco ; Fluhr, Joachim ; Cordiali-Fei, Paola. / Human herpesvirus 8 infection in patients with cutaneous lymphoproliferative diseases. In: Archives of Dermatology. 2005 ; Vol. 141, No. 10. pp. 1235-1242.
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abstract = "Objective: To investigate the prevalence of human herpesvirus 8 (HHV-8; Kaposi sarcoma-associated herpes-virus) infection in patients with lymphoproliferative skin diseases such as large-plaque parapsoriasis (LPP) and mycosis fungoides compared with inflammatory cutaneous conditions or healthy control subjects. Design: A survey study was undertaken in 123 subjects with various clinical conditions. Setting: All patients had been seen in the Dermatology Department of the San Gallicano Dermatology Institute, Rome, Italy, in the last 2 years. Patients: Forty-five patients with inflammatory or autoimmune cutaneous diseases, 50 healthy control subjects, 10 patients with LPP, 12 patients with mycosis fungoides, and 6 patients with classic Kaposi sarcoma were included in the study. Main Outcome Measures: The prevalence of HHV-8 infection was investigated with serologic studies using the gold standard assay based on body cavity-based B-cell lymphoma-1 cells latently infected with HHV-8. The presence of HHV-8 conserved sequence, corresponding to open reading frame 26, was also assessed in the peripheral blood and lesion tissue samples from patients with lymphoproliferative cutaneous diseases with nested polymerase chain reaction. The presence and distribution of cell types infected with HHV-8 in the lesion tissues was determined with immunohistochemical staining with the monoclonal antibody directed against the latent nuclear antigen-1 of HHV-8 encoded by open reading frame 73. Results: In healthy control subjects and patients with inflammatory skin diseases, 13.9{\%} were found to have antibody against HHV-8, consistent with the seroprevalence population in Italy. A highly significant association of HHV-8 infection and LPP was found (100{\%}) compared with mycosis fungoides (25{\%}). The peripheral blood mononuclear cells in 8 of 10 patients with LPP were found to harbor viral sequences at nested polymerase chain reaction, whereas none of them had a detectable serum viral load. All LPP lesion tissue samples were positive for HHV-8-encoded open reading frame 26, and the presence of HHV-8-infected cells was confirmed by immunohistochemistry profiles performed on paraffin-embedded tissues from 4 of 10 patients. The positive cell types included endothelial cells and the infiltrating dermal lymphocytes, characteristic hallmarks of LPP. Analysis of T-cell receptor γ chain rearrangements in lesion tissue from our patients confirmed the lack of a significant association between T-cell clonality and LPP. Conclusion: These data suggest that HHV-8 may play a role in the onset of LPP, a disease whose cause and evolution are still undefined and which has often been considered the early stage of mycosis fungoides.",
author = "Elisabetta Trento and Concetta Castilletti and Carmela Ferraro and {Lesnoni La Parola}, Ilaria and Anna Mussi and Luca Muscardin and Valentina Bordignon and Giovanna D'Agosto and Ada Amantea and Antonio Mastroianni and Franco Ameglio and Joachim Fluhr and Paola Cordiali-Fei",
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T1 - Human herpesvirus 8 infection in patients with cutaneous lymphoproliferative diseases

AU - Trento, Elisabetta

AU - Castilletti, Concetta

AU - Ferraro, Carmela

AU - Lesnoni La Parola, Ilaria

AU - Mussi, Anna

AU - Muscardin, Luca

AU - Bordignon, Valentina

AU - D'Agosto, Giovanna

AU - Amantea, Ada

AU - Mastroianni, Antonio

AU - Ameglio, Franco

AU - Fluhr, Joachim

AU - Cordiali-Fei, Paola

PY - 2005/10

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N2 - Objective: To investigate the prevalence of human herpesvirus 8 (HHV-8; Kaposi sarcoma-associated herpes-virus) infection in patients with lymphoproliferative skin diseases such as large-plaque parapsoriasis (LPP) and mycosis fungoides compared with inflammatory cutaneous conditions or healthy control subjects. Design: A survey study was undertaken in 123 subjects with various clinical conditions. Setting: All patients had been seen in the Dermatology Department of the San Gallicano Dermatology Institute, Rome, Italy, in the last 2 years. Patients: Forty-five patients with inflammatory or autoimmune cutaneous diseases, 50 healthy control subjects, 10 patients with LPP, 12 patients with mycosis fungoides, and 6 patients with classic Kaposi sarcoma were included in the study. Main Outcome Measures: The prevalence of HHV-8 infection was investigated with serologic studies using the gold standard assay based on body cavity-based B-cell lymphoma-1 cells latently infected with HHV-8. The presence of HHV-8 conserved sequence, corresponding to open reading frame 26, was also assessed in the peripheral blood and lesion tissue samples from patients with lymphoproliferative cutaneous diseases with nested polymerase chain reaction. The presence and distribution of cell types infected with HHV-8 in the lesion tissues was determined with immunohistochemical staining with the monoclonal antibody directed against the latent nuclear antigen-1 of HHV-8 encoded by open reading frame 73. Results: In healthy control subjects and patients with inflammatory skin diseases, 13.9% were found to have antibody against HHV-8, consistent with the seroprevalence population in Italy. A highly significant association of HHV-8 infection and LPP was found (100%) compared with mycosis fungoides (25%). The peripheral blood mononuclear cells in 8 of 10 patients with LPP were found to harbor viral sequences at nested polymerase chain reaction, whereas none of them had a detectable serum viral load. All LPP lesion tissue samples were positive for HHV-8-encoded open reading frame 26, and the presence of HHV-8-infected cells was confirmed by immunohistochemistry profiles performed on paraffin-embedded tissues from 4 of 10 patients. The positive cell types included endothelial cells and the infiltrating dermal lymphocytes, characteristic hallmarks of LPP. Analysis of T-cell receptor γ chain rearrangements in lesion tissue from our patients confirmed the lack of a significant association between T-cell clonality and LPP. Conclusion: These data suggest that HHV-8 may play a role in the onset of LPP, a disease whose cause and evolution are still undefined and which has often been considered the early stage of mycosis fungoides.

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