Human HLA-DRα gene

a rare oligonucleotide (GTATA) identifies an upstream sequence required for nuclear protein binding

Rafaella Barbieri, Patrizio Giacomini, Stefano Volinia, Claudio Nastruzzi, Anna Maria Mileo, Umberto Ferrini, Marco Soria, Italo Barrai, Pier Giorgio Natali, Roberto Gambari

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Synthetic oligonucleotides containing putative regulatory sequences are currently employed to identify and isolate genes coding for nuclear binding factors. Upstream DNA sequences of eukaryotic genes required for transcriptional activity and tissue specificity can be identified by means of biochemical techniques as well as computer analysis using homology searching. An alternative approach has been recently proposed by our research group. Scanning DNA sequences 1.8 megabases in length from a Genetic Sequence Data Bank, we have identified rare oligonucleotides 5 base pairs (bp) long, which are localized within or close to regulatory segments in mammalian promoters. In this paper we demonstrate that the rare GTATA sequence identifies an upstream region of the HLA-DRα gene which operates in conjunction with the sequence AGAAGTCAG, homologous to a box found in many interferon-inducible genes, in binding nuclear proteins.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalFEBS Letters
Volume268
Issue number1
DOIs
Publication statusPublished - Jul 30 1990

Fingerprint

HLA-DR Antigens
Nuclear Proteins
Protein Binding
Oligonucleotides
Genes
DNA sequences
Genetic Databases
Organ Specificity
Sequence Homology
Base Pairing
Interferons
Carrier Proteins
Tissue
Scanning
Research

Keywords

  • DNA-binding factor
  • HLA-DRα
  • Oligonucleotide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Cite this

Human HLA-DRα gene : a rare oligonucleotide (GTATA) identifies an upstream sequence required for nuclear protein binding. / Barbieri, Rafaella; Giacomini, Patrizio; Volinia, Stefano; Nastruzzi, Claudio; Mileo, Anna Maria; Ferrini, Umberto; Soria, Marco; Barrai, Italo; Natali, Pier Giorgio; Gambari, Roberto.

In: FEBS Letters, Vol. 268, No. 1, 30.07.1990, p. 51-54.

Research output: Contribution to journalArticle

Barbieri, R, Giacomini, P, Volinia, S, Nastruzzi, C, Mileo, AM, Ferrini, U, Soria, M, Barrai, I, Natali, PG & Gambari, R 1990, 'Human HLA-DRα gene: a rare oligonucleotide (GTATA) identifies an upstream sequence required for nuclear protein binding', FEBS Letters, vol. 268, no. 1, pp. 51-54. https://doi.org/10.1016/0014-5793(90)80970-T
Barbieri, Rafaella ; Giacomini, Patrizio ; Volinia, Stefano ; Nastruzzi, Claudio ; Mileo, Anna Maria ; Ferrini, Umberto ; Soria, Marco ; Barrai, Italo ; Natali, Pier Giorgio ; Gambari, Roberto. / Human HLA-DRα gene : a rare oligonucleotide (GTATA) identifies an upstream sequence required for nuclear protein binding. In: FEBS Letters. 1990 ; Vol. 268, No. 1. pp. 51-54.
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