Human immunodeficiency virus-induced cell death in cytokine-treated macrophages can be prevented by compounds that inhibit late stages of viral replication

Alberto Bergamini, Luciana Dini, Marcella Capozzi, Lina Ghibelli, Roberta Placido, Emanuela Faggioli, Agata Salanitro, Elena Buonanno, Luisa Cappannoli, Laura Ventura, Mario Cepparulo, Laura Falasca, Giovanni Rocchi

Research output: Contribution to journalArticle

Abstract

The basis of the cytopathic effect induced by a laboratory strain and several clinical isolates of human immunodeficiency virus (HIV) in human macrophages cultured in the presence of macrophage colony-stimulating factor was studied. Infected macrophages die of necrosis, the consequence of the production of mature virions in infected cells. Cell death can be prevented by antiviral compounds that interfere with the assembly and budding of virions. Programmed cell death (apoptosis), a potential mechanism of HIV- mediated cell death in CD4 T lymphocytes, does not occur in infected macrophages as shown by electron microscopy, cytofluorometric and gel electrophoretic DNA analysis, and nuclear fluorescent staining by Hoechst and terminal dUTP-nick-end-labeling (TUNEL) assay. The data suggest that macrophage killing by HIV may occur in vivo. Thus, combination therapies that include compounds that inhibit the cytopathic effect of HIV in macrophages should be considered for AIDS patients.

Original languageEnglish
Pages (from-to)1367-1378
Number of pages12
JournalJournal of Infectious Diseases
Volume173
Issue number6
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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    Bergamini, A., Dini, L., Capozzi, M., Ghibelli, L., Placido, R., Faggioli, E., Salanitro, A., Buonanno, E., Cappannoli, L., Ventura, L., Cepparulo, M., Falasca, L., & Rocchi, G. (1996). Human immunodeficiency virus-induced cell death in cytokine-treated macrophages can be prevented by compounds that inhibit late stages of viral replication. Journal of Infectious Diseases, 173(6), 1367-1378.