Abstract
A broad and rapidly changing HIV Type 1 (HIV-1) diversity has been reported from different populations in Cameroon since the early epidemic. Our understanding of HIV-1 dynamics can be improved by a systematic surveillance in Cameroon as accessibility and use of antiretroviral drugs increase. To contribute to this, we genotyped 30 samples by sequencing the protease and reverse transcriptase (proRT) genes of HIV-1. Phylogenetic analysis of the HIV-1 proRT sequences using the MEGA3 software showed that 26 (86.7%) were recombinant forms which included 20 (66.7%) circulating recombinant forms: CRF02-AG, (50%), CRF06-cpx (3.3%), CRF11-cpx (10%) and CRF37-cpx (3.3%), and 6 unique recombinant forms (URF, 20%). Two of the six URFs were second generation recombinants and 4 contained unclassified segments. HIV-1 subtypes Al (3.3%), C (3.3%) and D (6.7%) were also identified. Although partial sequences of HIV-1 genome were analysed, our results indicate that recombinant HIV-1 variants predominate in the AIDS epidemic in Cameroon. With the widespread use of antiretroviral drugs in Cameroon and the circulation of several HIV-1 variants within this population, the emergence of recombinants with unknown diagnostic and clinical consequences is a concern.
| Original language | English |
|---|---|
| Pages (from-to) | 325-332 |
| Number of pages | 8 |
| Journal | New Microbiologica |
| Volume | 32 |
| Issue number | 4 |
| Publication status | Published - Oct 2009 |
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Keywords
- Cameroon
- Diversity
- HIV
- Recombinant forms
ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Human immunodeficiency virus type 1 intersubtype recombinants predominate in the AIDS epidemic in Cameroon. / Torimiro, Judith N.; D'Arrigo, Roberta; Takou, Desire; Nanfack, Aubin; Pizzi, Daniele; Ngong, Innocent; Carr, Jean K.; Joseph, Fouda Pierre; Perno, Carlo Federico; Cappelli, Giulia.
In: New Microbiologica, Vol. 32, No. 4, 10.2009, p. 325-332.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human immunodeficiency virus type 1 intersubtype recombinants predominate in the AIDS epidemic in Cameroon
AU - Torimiro, Judith N.
AU - D'Arrigo, Roberta
AU - Takou, Desire
AU - Nanfack, Aubin
AU - Pizzi, Daniele
AU - Ngong, Innocent
AU - Carr, Jean K.
AU - Joseph, Fouda Pierre
AU - Perno, Carlo Federico
AU - Cappelli, Giulia
PY - 2009/10
Y1 - 2009/10
N2 - A broad and rapidly changing HIV Type 1 (HIV-1) diversity has been reported from different populations in Cameroon since the early epidemic. Our understanding of HIV-1 dynamics can be improved by a systematic surveillance in Cameroon as accessibility and use of antiretroviral drugs increase. To contribute to this, we genotyped 30 samples by sequencing the protease and reverse transcriptase (proRT) genes of HIV-1. Phylogenetic analysis of the HIV-1 proRT sequences using the MEGA3 software showed that 26 (86.7%) were recombinant forms which included 20 (66.7%) circulating recombinant forms: CRF02-AG, (50%), CRF06-cpx (3.3%), CRF11-cpx (10%) and CRF37-cpx (3.3%), and 6 unique recombinant forms (URF, 20%). Two of the six URFs were second generation recombinants and 4 contained unclassified segments. HIV-1 subtypes Al (3.3%), C (3.3%) and D (6.7%) were also identified. Although partial sequences of HIV-1 genome were analysed, our results indicate that recombinant HIV-1 variants predominate in the AIDS epidemic in Cameroon. With the widespread use of antiretroviral drugs in Cameroon and the circulation of several HIV-1 variants within this population, the emergence of recombinants with unknown diagnostic and clinical consequences is a concern.
AB - A broad and rapidly changing HIV Type 1 (HIV-1) diversity has been reported from different populations in Cameroon since the early epidemic. Our understanding of HIV-1 dynamics can be improved by a systematic surveillance in Cameroon as accessibility and use of antiretroviral drugs increase. To contribute to this, we genotyped 30 samples by sequencing the protease and reverse transcriptase (proRT) genes of HIV-1. Phylogenetic analysis of the HIV-1 proRT sequences using the MEGA3 software showed that 26 (86.7%) were recombinant forms which included 20 (66.7%) circulating recombinant forms: CRF02-AG, (50%), CRF06-cpx (3.3%), CRF11-cpx (10%) and CRF37-cpx (3.3%), and 6 unique recombinant forms (URF, 20%). Two of the six URFs were second generation recombinants and 4 contained unclassified segments. HIV-1 subtypes Al (3.3%), C (3.3%) and D (6.7%) were also identified. Although partial sequences of HIV-1 genome were analysed, our results indicate that recombinant HIV-1 variants predominate in the AIDS epidemic in Cameroon. With the widespread use of antiretroviral drugs in Cameroon and the circulation of several HIV-1 variants within this population, the emergence of recombinants with unknown diagnostic and clinical consequences is a concern.
KW - Cameroon
KW - Diversity
KW - HIV
KW - Recombinant forms
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M3 - Article
C2 - 20128438
AN - SCOPUS:74249113795
VL - 32
SP - 325
EP - 332
JO - New Microbiologica
JF - New Microbiologica
SN - 1121-7138
IS - 4
ER -