Human immunodeficient virus type 1 gp120 C5 region mimics the HLA class I α1 peptide-binding domain

L. Lopalco, C. De Santis, R. Meneveri, R. Longhi, E. Ginelli, F. Grassi, A. G. Siccardi, A. Beretta

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Molecular mimicry of major histocompatibility (MHC) antigens by viral glyco-proteins has been suggested as one of the possible mechanisms of induction of an autoimmnune response by human immunodeficiency viruses. A monoclonal antibody (M38) was previously shown to bind to both human immunodeficiency virus type 1 (HIV-1) gp120 and β-2 microglobulin-free HLA class I heavy chains encoded by an HLA C allele. Using HLA C recombinant proteins and synthetic peptides, the M38 class I binding site was mapped to a stretch of 44 amino acids of the α1 domain. The amino acid residues recognized are clustered in two non-contiguous regions at positions 66-69 (KYKR) and 79-82 (RKLR) shared by almost all HLA C alleles. On HIV-1 gp120, M38 binds to two non-contiguous sequences (KYK and KAKR) at positions 490-492 and 505-508 located at the edges of a large hydrophobic region that is apparently involved in binding the transmembrane glycoprotein gp41. The C-terminal gp120 M38-reactive region (KAKR) lies within the immunodominant sequence APTKAKRRVVQREKR, against which the majority of HIV-infected individulals produce antibodies. The results indicate that a functionally important region of HIV-1 gp120 shares similar amino acid sequence motifs with the antigen recognition site of most HLA class I C alleles. The molecular mimicry may be the basis for autoimmune responses in HIV infection.

Original languageEnglish
Pages (from-to)2016-2021
Number of pages6
JournalEuropean Journal of Immunology
Volume23
Issue number8
Publication statusPublished - 1993

Fingerprint

HLA-C Antigens
Molecular Mimicry
HIV-1
Alleles
Viruses
HIV
Amino Acids
Amino Acid Motifs
Histocompatibility Antigens
Viral Proteins
Autoimmunity
Recombinant Proteins
HIV Infections
Amino Acid Sequence
Glycoproteins
Binding Sites
Monoclonal Antibodies
Antigens
Antibodies
Protein Domains

Keywords

  • Acquired immunodeficiency syndrome
  • Autoimmunity
  • HLA
  • Human immunodeficiency virus

ASJC Scopus subject areas

  • Immunology

Cite this

Lopalco, L., De Santis, C., Meneveri, R., Longhi, R., Ginelli, E., Grassi, F., ... Beretta, A. (1993). Human immunodeficient virus type 1 gp120 C5 region mimics the HLA class I α1 peptide-binding domain. European Journal of Immunology, 23(8), 2016-2021.

Human immunodeficient virus type 1 gp120 C5 region mimics the HLA class I α1 peptide-binding domain. / Lopalco, L.; De Santis, C.; Meneveri, R.; Longhi, R.; Ginelli, E.; Grassi, F.; Siccardi, A. G.; Beretta, A.

In: European Journal of Immunology, Vol. 23, No. 8, 1993, p. 2016-2021.

Research output: Contribution to journalArticle

Lopalco, L, De Santis, C, Meneveri, R, Longhi, R, Ginelli, E, Grassi, F, Siccardi, AG & Beretta, A 1993, 'Human immunodeficient virus type 1 gp120 C5 region mimics the HLA class I α1 peptide-binding domain', European Journal of Immunology, vol. 23, no. 8, pp. 2016-2021.
Lopalco, L. ; De Santis, C. ; Meneveri, R. ; Longhi, R. ; Ginelli, E. ; Grassi, F. ; Siccardi, A. G. ; Beretta, A. / Human immunodeficient virus type 1 gp120 C5 region mimics the HLA class I α1 peptide-binding domain. In: European Journal of Immunology. 1993 ; Vol. 23, No. 8. pp. 2016-2021.
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