Human intestinal epithelial cells promote the differentiation of tolerogenic dendritic cells

I. D. Iliev, I. Spadoni, E. Mileti, G. Matteoli, A. Sonzogni, G. M. Sampietro, D. Foschi, F. Caprioli, G. Viale, M. Rescigno

Research output: Contribution to journalArticlepeer-review


Objective: In mice, a subpopulation of gut dendritic cells (DCs) expressing CD103 drives the development of regulatory T (Treg) cells. Further, it was recently described that the cross-talk between human intestinal epithelial cells (IECs) and DCs helps in maintaining gut immune homeostasis via the induction of non-inflammatory DCs. In this study, an analysis was carried out to determine whether IECs could promote the differentiation of CD103 + tolerogenic DCs, and the function of primary CD103+ DCs isolated from human mesenteric lymph nodes (MLNs) was evaluated. Methods: Monocyte-derived DCs (MoDCs) and circulating CD1c+ DCs were conditioned or not with supernatants from Caco-2 cells or IECs isolated from healthy donors or donors with Crohn's disease and analysed for their ability to induce Treg cell differentiation. In some cases, transforming growth factor β (TGFb), retinoic acid (RA) or thymic stromal lymphopoietin (TSLP) were neutralised before conditioning. CD103+ and CD1032 DCs were sorted by fluorescence-activated cell sorting (FACS) from MLNs and used in Treg cell differentiation experiments. Results: It was found that human IECs promoted the differentiation of tolerogenic DCs able to drive the development of adaptive Foxp3+ Treg cells. This control was lost in patients with Crohn's disease and paralleled a reduced expression of tolerogenic factors by primary IECs. MoDCs differentiated with RA or IEC supernatant upregulated the expression of CD103. Consistently, human primary CD103+ DCs isolated from MLNs were endowed with the ability to drive Treg cell differentiation. This subset of DCs expressed CCR7 and probably represents a lamina propria-derived migratory population. Conclusions: A population of tolerogenic CD103+ DCs was identified in the human gut that probably differentiate in response to IEC-derived factors and drive Treg cell development.

Original languageEnglish
Pages (from-to)1481-1489
Number of pages9
Issue number11
Publication statusPublished - Nov 2009

ASJC Scopus subject areas

  • Gastroenterology


Dive into the research topics of 'Human intestinal epithelial cells promote the differentiation of tolerogenic dendritic cells'. Together they form a unique fingerprint.

Cite this