TY - JOUR
T1 - Human leucocyte antigen I expression in spermatozoa from infertile men
AU - Paradisi, Roberto
AU - Neri, Simona
AU - Pession, Annalisa
AU - Magrini, Elisabetta
AU - Bellavia, Emanuela
AU - Ceccardi, Stefania
AU - Venturoli, Stefano
AU - Flamigni, Carlo
PY - 2001
Y1 - 2001
N2 - The expression of class I human leucocyte antigen (HLA) has been investigated by reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry on human purified ejaculated spermatozoa from an infertile population. Similar investigation in a control fertile population has been performed. Among 22 fertile and 20 infertile men, 17 were selected for the study because they showed no contamination with non-sperm cells. HLA I molecules were expressed in four of nine fertile subjects (44.4%) and three of eight infertile subjects (37.5%), with no significant difference between groups. These data demonstrate for the first time the presence of class I HLA antigens on spermatozoa of infertile subjects. In addition, considering that similar results have been obtained in both fertile and infertile populations, the hypothesis that the fertilizing capacity of the spermatozoon is independent of class I HLA-antigen expression on their surface may be advanced.
AB - The expression of class I human leucocyte antigen (HLA) has been investigated by reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry on human purified ejaculated spermatozoa from an infertile population. Similar investigation in a control fertile population has been performed. Among 22 fertile and 20 infertile men, 17 were selected for the study because they showed no contamination with non-sperm cells. HLA I molecules were expressed in four of nine fertile subjects (44.4%) and three of eight infertile subjects (37.5%), with no significant difference between groups. These data demonstrate for the first time the presence of class I HLA antigens on spermatozoa of infertile subjects. In addition, considering that similar results have been obtained in both fertile and infertile populations, the hypothesis that the fertilizing capacity of the spermatozoon is independent of class I HLA-antigen expression on their surface may be advanced.
KW - Class I human leucocyte antigens
KW - Flow cytometry
KW - Male infertility
KW - Reverse transcription-polymerase chain reaction
KW - Spermatozoa
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U2 - 10.1046/j.1365-2605.2001.00256.x
DO - 10.1046/j.1365-2605.2001.00256.x
M3 - Article
C2 - 11168645
AN - SCOPUS:0035136210
VL - 24
SP - 8
EP - 14
JO - International Journal of Andrology
JF - International Journal of Andrology
SN - 0105-6263
IS - 1
ER -