Human leukemia cells synthesize and secrete proteins related to platelet-derived growth factor

P. Pantazis, L. Lanfrancone, P. G. Pelicci, R. Dalla-Favera, H. N. Antoniades

Research output: Contribution to journalArticle

Abstract

Human leukemia cells in culture (HL-60) synthesize and secrete proteins that are recognized by antiserum to human platelet-derived growth factor (PDGF). The molecular mass of the intracellular proteins immunoprecipitated by PDGF antiserum ranged from 34 kDa to 240 kDa. PDGF-related proteins were also identified in the conditioned medium of the cells. Several of these immunoprecipitated proteins were glycosylated. A single protein of 46 kDa was immunoprecipitated from the cell-free translation products of mRNA obtained from the leukemia cells. Antiserum to the C but not to the N terminus of the predicted amino acid sequence of the transforming protein p28(sis)/PDGF-2 also immunoprecipitated proteins secreted by the HL-60 cells. These findings provide a direct demonstration for the synthesis and secretion of PDGF-like proteins by leukemia cells in culture. These proteins do not appear to be coded by the known c-sis/PDGF-2 locus since no sis mRNA was detectable in the HL-60 cells.

Original languageEnglish
Pages (from-to)5526-5530
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number15
DOIs
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Genetics
  • General

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