Abstract
In this article we show that the cytidine analog 5-azacytidine is able to induce differentiation of the human leukemia K-562 cell line. Erythroid induction is associated with (a) an increase of the overall globin synthesis and globin mRNA accumulation, (b) a relative increase of fetal with respect to embryonic globins, and (c) a decrease of the proliferative capacity of hemoglobin-containing cells. In addition, we have analysed the DNA methylation pattern at the cleavage sites of MspI and HpaII restriction enzymes, which are known to cleave differently CCGG DNA sequences when 5-methylcytosine is present. These experiments indicate that in K-562 cells treated with 5-azacytidine, DNA becomes hypomethylated, suggesting that genetic programmes leading to an erythroid phenotype may be activated by a reduction of DNA methylation.
| Original language | English |
|---|---|
| Pages (from-to) | 87-97 |
| Number of pages | 11 |
| Journal | Cell Differentiation |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1984 |
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Keywords
- 5-azacytidine
- DNA methylation
- erythroid differentiation
- globin mRNA
- K-562 cell
ASJC Scopus subject areas
- Developmental Biology
Cite this
Human leukemia K-562 cells : induction of erythroid differentiation by 5-azacytidine. / Gambari, Roberto; del Senno, Laura; Barbieri, Raffaella; Viola, Lorenza; Tripodi, Marco; Raschellà, Giuseppe; Fantoni, Antonio.
In: Cell Differentiation, Vol. 14, No. 2, 1984, p. 87-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human leukemia K-562 cells
T2 - induction of erythroid differentiation by 5-azacytidine
AU - Gambari, Roberto
AU - del Senno, Laura
AU - Barbieri, Raffaella
AU - Viola, Lorenza
AU - Tripodi, Marco
AU - Raschellà, Giuseppe
AU - Fantoni, Antonio
PY - 1984
Y1 - 1984
N2 - In this article we show that the cytidine analog 5-azacytidine is able to induce differentiation of the human leukemia K-562 cell line. Erythroid induction is associated with (a) an increase of the overall globin synthesis and globin mRNA accumulation, (b) a relative increase of fetal with respect to embryonic globins, and (c) a decrease of the proliferative capacity of hemoglobin-containing cells. In addition, we have analysed the DNA methylation pattern at the cleavage sites of MspI and HpaII restriction enzymes, which are known to cleave differently CCGG DNA sequences when 5-methylcytosine is present. These experiments indicate that in K-562 cells treated with 5-azacytidine, DNA becomes hypomethylated, suggesting that genetic programmes leading to an erythroid phenotype may be activated by a reduction of DNA methylation.
AB - In this article we show that the cytidine analog 5-azacytidine is able to induce differentiation of the human leukemia K-562 cell line. Erythroid induction is associated with (a) an increase of the overall globin synthesis and globin mRNA accumulation, (b) a relative increase of fetal with respect to embryonic globins, and (c) a decrease of the proliferative capacity of hemoglobin-containing cells. In addition, we have analysed the DNA methylation pattern at the cleavage sites of MspI and HpaII restriction enzymes, which are known to cleave differently CCGG DNA sequences when 5-methylcytosine is present. These experiments indicate that in K-562 cells treated with 5-azacytidine, DNA becomes hypomethylated, suggesting that genetic programmes leading to an erythroid phenotype may be activated by a reduction of DNA methylation.
KW - 5-azacytidine
KW - DNA methylation
KW - erythroid differentiation
KW - globin mRNA
KW - K-562 cell
UR - http://www.scopus.com/inward/record.url?scp=0021215472&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021215472&partnerID=8YFLogxK
U2 - 10.1016/0045-6039(84)90033-2
DO - 10.1016/0045-6039(84)90033-2
M3 - Article
C2 - 6205767
AN - SCOPUS:0021215472
VL - 14
SP - 87
EP - 97
JO - Cell Differentiation
JF - Cell Differentiation
SN - 0045-6039
IS - 2
ER -