Human leukocyte antigen II expression in sperm cells: Comparison between fertile and infertile men

R. Paradisi, S. Neri, A. Pession, E. Magrini, E. Bellavia, S. Ceccardi, C. Flamigni

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Human leukocyte antigens (HLA) class II transcripts in mature spermatozoa of healthy volunteers have recently been demonstrated using reverse transcription-polymerase chain reaction (RT-PCR). HLA II expression was investigated on ejaculated sperm cells in fertile and infertile men by RT-PCR and flow cytometry. Among 22 fertile and 20 infertile men, 18 were selected for the study because they showed no contamination with non-sperm cells. HLA II mRNA transcripts were expressed in all but 1 of 8 infertile subjects and in only 2 of 10 fertile ones. The cytofluorometric analysis on three RT-PCR positive samples confirmed the presence of class II antigens on cell surfaces. These data clearly confirm the presence of both HLA II mRNA and surface molecules on human sperm cells. In addition, an interesting nonrandom distribution of positivity among fertile and infertile samples regarding HLA II expression (p <. 025) suggests a possible correlation with infertility.

Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalSystems Biology in Reproductive Medicine
Volume45
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

HLA Antigens
Spermatozoa
Reverse Transcription
Polymerase Chain Reaction
Messenger RNA
Histocompatibility Antigens Class II
Infertility
Healthy Volunteers
Flow Cytometry

Keywords

  • flow cytometry
  • HLA class II antigen
  • male infertility
  • reverse transcriptionpolymerase chain reaction
  • spermatozoa

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

Cite this

Human leukocyte antigen II expression in sperm cells : Comparison between fertile and infertile men. / Paradisi, R.; Neri, S.; Pession, A.; Magrini, E.; Bellavia, E.; Ceccardi, S.; Flamigni, C.

In: Systems Biology in Reproductive Medicine, Vol. 45, No. 3, 2000, p. 203-213.

Research output: Contribution to journalArticle

Paradisi, R. ; Neri, S. ; Pession, A. ; Magrini, E. ; Bellavia, E. ; Ceccardi, S. ; Flamigni, C. / Human leukocyte antigen II expression in sperm cells : Comparison between fertile and infertile men. In: Systems Biology in Reproductive Medicine. 2000 ; Vol. 45, No. 3. pp. 203-213.
@article{e9aa3f98848345ec999f9f848afdb704,
title = "Human leukocyte antigen II expression in sperm cells: Comparison between fertile and infertile men",
abstract = "Human leukocyte antigens (HLA) class II transcripts in mature spermatozoa of healthy volunteers have recently been demonstrated using reverse transcription-polymerase chain reaction (RT-PCR). HLA II expression was investigated on ejaculated sperm cells in fertile and infertile men by RT-PCR and flow cytometry. Among 22 fertile and 20 infertile men, 18 were selected for the study because they showed no contamination with non-sperm cells. HLA II mRNA transcripts were expressed in all but 1 of 8 infertile subjects and in only 2 of 10 fertile ones. The cytofluorometric analysis on three RT-PCR positive samples confirmed the presence of class II antigens on cell surfaces. These data clearly confirm the presence of both HLA II mRNA and surface molecules on human sperm cells. In addition, an interesting nonrandom distribution of positivity among fertile and infertile samples regarding HLA II expression (p <. 025) suggests a possible correlation with infertility.",
keywords = "flow cytometry, HLA class II antigen, male infertility, reverse transcriptionpolymerase chain reaction, spermatozoa",
author = "R. Paradisi and S. Neri and A. Pession and E. Magrini and E. Bellavia and S. Ceccardi and C. Flamigni",
year = "2000",
doi = "10.1080/01485010050193986",
language = "English",
volume = "45",
pages = "203--213",
journal = "Systems Biology in Reproductive Medicine",
issn = "1939-6368",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Human leukocyte antigen II expression in sperm cells

T2 - Comparison between fertile and infertile men

AU - Paradisi, R.

AU - Neri, S.

AU - Pession, A.

AU - Magrini, E.

AU - Bellavia, E.

AU - Ceccardi, S.

AU - Flamigni, C.

PY - 2000

Y1 - 2000

N2 - Human leukocyte antigens (HLA) class II transcripts in mature spermatozoa of healthy volunteers have recently been demonstrated using reverse transcription-polymerase chain reaction (RT-PCR). HLA II expression was investigated on ejaculated sperm cells in fertile and infertile men by RT-PCR and flow cytometry. Among 22 fertile and 20 infertile men, 18 were selected for the study because they showed no contamination with non-sperm cells. HLA II mRNA transcripts were expressed in all but 1 of 8 infertile subjects and in only 2 of 10 fertile ones. The cytofluorometric analysis on three RT-PCR positive samples confirmed the presence of class II antigens on cell surfaces. These data clearly confirm the presence of both HLA II mRNA and surface molecules on human sperm cells. In addition, an interesting nonrandom distribution of positivity among fertile and infertile samples regarding HLA II expression (p <. 025) suggests a possible correlation with infertility.

AB - Human leukocyte antigens (HLA) class II transcripts in mature spermatozoa of healthy volunteers have recently been demonstrated using reverse transcription-polymerase chain reaction (RT-PCR). HLA II expression was investigated on ejaculated sperm cells in fertile and infertile men by RT-PCR and flow cytometry. Among 22 fertile and 20 infertile men, 18 were selected for the study because they showed no contamination with non-sperm cells. HLA II mRNA transcripts were expressed in all but 1 of 8 infertile subjects and in only 2 of 10 fertile ones. The cytofluorometric analysis on three RT-PCR positive samples confirmed the presence of class II antigens on cell surfaces. These data clearly confirm the presence of both HLA II mRNA and surface molecules on human sperm cells. In addition, an interesting nonrandom distribution of positivity among fertile and infertile samples regarding HLA II expression (p <. 025) suggests a possible correlation with infertility.

KW - flow cytometry

KW - HLA class II antigen

KW - male infertility

KW - reverse transcriptionpolymerase chain reaction

KW - spermatozoa

UR - http://www.scopus.com/inward/record.url?scp=79961203465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79961203465&partnerID=8YFLogxK

U2 - 10.1080/01485010050193986

DO - 10.1080/01485010050193986

M3 - Article

AN - SCOPUS:79961203465

VL - 45

SP - 203

EP - 213

JO - Systems Biology in Reproductive Medicine

JF - Systems Biology in Reproductive Medicine

SN - 1939-6368

IS - 3

ER -