TY - JOUR
T1 - Human lymphocytes assay
T2 - Cyclophosphamide metabolic activation by S9 system with low cytotoxicity
AU - Sbrana, I.
AU - Zaccaro, L.
AU - Lascialfari, D.
AU - Ceccherini, I.
AU - Loprieno, N.
PY - 1984
Y1 - 1984
N2 - The general suitability of exposing human lymphocytes directly to prolonged contact with an Ames-type microsomal (S9) activation system has been examined, for testing the effect of the indirect chemical mutagen, cyclophosphamide (CPA), on induction of chromosomal aberrations. Direct exposure of lymphocytes to only S9 mix produced a decrease in the mitotic index within 30-60 min, whereafter it stabilized at acceptable values. Further toxic effects following treatment with different doses of CPA and S9 mix, for the longest times of exposure were due to production of clastogenic metabolites. On the basis of these result, the low cytotoxicity of S9 mix in our conditions allows extension of the application of the test to the study of metabolites which require prolonged contact with the target cells.
AB - The general suitability of exposing human lymphocytes directly to prolonged contact with an Ames-type microsomal (S9) activation system has been examined, for testing the effect of the indirect chemical mutagen, cyclophosphamide (CPA), on induction of chromosomal aberrations. Direct exposure of lymphocytes to only S9 mix produced a decrease in the mitotic index within 30-60 min, whereafter it stabilized at acceptable values. Further toxic effects following treatment with different doses of CPA and S9 mix, for the longest times of exposure were due to production of clastogenic metabolites. On the basis of these result, the low cytotoxicity of S9 mix in our conditions allows extension of the application of the test to the study of metabolites which require prolonged contact with the target cells.
UR - http://www.scopus.com/inward/record.url?scp=0021674070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021674070&partnerID=8YFLogxK
U2 - 10.1016/0165-1161(84)90013-X
DO - 10.1016/0165-1161(84)90013-X
M3 - Article
C2 - 6513968
AN - SCOPUS:0021674070
VL - 130
SP - 411
EP - 416
JO - Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology
JF - Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology
SN - 0165-1161
IS - 6
ER -