TY - JOUR
T1 - Human mast cells take up and hydrolyze anandamide under the control of 5-lipoxygenase and do not express cannabinoid receptors
AU - MacCarrone, Mauro
AU - Fiorucci, Laura
AU - Erba, Fulvio
AU - Bari, Monica
AU - Finazzi-Agrò, Alessandro
AU - Ascoli, Franca
PY - 2000/2/25
Y1 - 2000/2/25
N2 - Human mast cells (HMC-1) take up anandamide (arachidonoyl-ethanolamide, AEA) with a saturable process (K(m) = 200±20 nM, V(max) = 25±3 pmol min-1 mg protein-1), enhanced two-fold over control by nitric oxide-donors. Internalized AEA was hydrolyzed by a fatty acid amide hydrolase (FAAH), whose activity became measurable only in the presence of 5-lipoxygenase, but not cyclooxygenase, inhibitors. FAAH (K(m) = 5.0±0.5 μM, V(max) = 160±15 pmol min-1 mg protein-1) was competitively inhibited by palmitoylethanolamide. HMC-1 cells did not display a functional cannabinoid receptor on their surface and neither AEA nor palmitoylethanolamide affected tryptase release from these cells. (C) 2000 Federation of European Biochemical Societies.
AB - Human mast cells (HMC-1) take up anandamide (arachidonoyl-ethanolamide, AEA) with a saturable process (K(m) = 200±20 nM, V(max) = 25±3 pmol min-1 mg protein-1), enhanced two-fold over control by nitric oxide-donors. Internalized AEA was hydrolyzed by a fatty acid amide hydrolase (FAAH), whose activity became measurable only in the presence of 5-lipoxygenase, but not cyclooxygenase, inhibitors. FAAH (K(m) = 5.0±0.5 μM, V(max) = 160±15 pmol min-1 mg protein-1) was competitively inhibited by palmitoylethanolamide. HMC-1 cells did not display a functional cannabinoid receptor on their surface and neither AEA nor palmitoylethanolamide affected tryptase release from these cells. (C) 2000 Federation of European Biochemical Societies.
KW - Anandamide
KW - Endocannabinoid
KW - Inflammation
KW - Lipoxygenase
KW - Nitric oxide
KW - Tryptase
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U2 - 10.1016/S0014-5793(00)01223-0
DO - 10.1016/S0014-5793(00)01223-0
M3 - Article
C2 - 10692582
AN - SCOPUS:0033961214
VL - 468
SP - 176
EP - 180
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -