Human melanoma cells express a functional interleukin-2 receptor

S. Plaisance, E. Rubinstein, A. Alileche, D. S. Han, Y. Sahraoui, M. C. Mingari, R. Bellomo, D. Rimoldi, M. P. Colombo, C. Jasmin, S. Carrel, B. Azzarone

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Abstract

Flow cytometric analysis reveals that 5 human melanoma cell lines (M14, IGR3, ME1477, JUSO, GLL19) express both α and β chain of the interleukin 2 receptor (IL-2Rα and IL-2Rβ). These chains are able to specifically bind IL-2 and to form high-affinity heterodimers (IL-2Rαβ). Analysis of poly A+ RNAs by Northern blot reveals the presence of typical transcripts for both the IL-2Rα gene (3.6 kb) and the IL-2Rβ gene (4 kb). Reverse transcription/polymerase chain reaction analysis allowed transcripts for the IL-2Rγ (p64) gene to be detected in 3 of these melanoma cell lines (M14, IGR3, ME1477). Incubation with human recombinant IL-2 modifies in IL-2Rα+β+γ+ (M14) the expression of several surface molecules: down-regulation of ICAM-1, HLA class I and HLA-DR and up-regulation of CD44. IL-2 is also active on IL-2α+β+γ- cell lines since it decreases ICAM-1 and HLA class-II expression at the surface of JUSO cells. Down-regulation of ICAM-1, whose expression in melanoma cells is a marker of tumor progression, is detectable within 3 hr in M14 cells and is maximal after 48 hr incubation, at IL-2 concentrations corresponding to the high-affinity heterodimers. This feature is specific since it is partially inhibited by MAbs directed against the IL-2 binding site of the IL-2Rα (MAR93, I0T14) and IL-2Rβ (MiKβ1, TU27) chains. Our data support the notion of a direct effect of IL-2 on human melanoma cells. Modulation of the expression of surface molecules which is important for the interaction with immunocompetent cells or for tumor progression, could have a role to play during in vivo IL-2 treatment of human melanomas.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalInternational Journal of Cancer
Volume55
Issue number1
Publication statusPublished - 1993

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Interleukin-2 Receptors
Interleukin-2
Melanoma
Intercellular Adhesion Molecule-1
Cell Line
Down-Regulation
Genes
HLA-DR Antigens
Tumor Biomarkers
Northern Blotting
Reverse Transcription
Up-Regulation
Binding Sites
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Plaisance, S., Rubinstein, E., Alileche, A., Han, D. S., Sahraoui, Y., Mingari, M. C., ... Azzarone, B. (1993). Human melanoma cells express a functional interleukin-2 receptor. International Journal of Cancer, 55(1), 164-170.

Human melanoma cells express a functional interleukin-2 receptor. / Plaisance, S.; Rubinstein, E.; Alileche, A.; Han, D. S.; Sahraoui, Y.; Mingari, M. C.; Bellomo, R.; Rimoldi, D.; Colombo, M. P.; Jasmin, C.; Carrel, S.; Azzarone, B.

In: International Journal of Cancer, Vol. 55, No. 1, 1993, p. 164-170.

Research output: Contribution to journalArticle

Plaisance, S, Rubinstein, E, Alileche, A, Han, DS, Sahraoui, Y, Mingari, MC, Bellomo, R, Rimoldi, D, Colombo, MP, Jasmin, C, Carrel, S & Azzarone, B 1993, 'Human melanoma cells express a functional interleukin-2 receptor', International Journal of Cancer, vol. 55, no. 1, pp. 164-170.
Plaisance S, Rubinstein E, Alileche A, Han DS, Sahraoui Y, Mingari MC et al. Human melanoma cells express a functional interleukin-2 receptor. International Journal of Cancer. 1993;55(1):164-170.
Plaisance, S. ; Rubinstein, E. ; Alileche, A. ; Han, D. S. ; Sahraoui, Y. ; Mingari, M. C. ; Bellomo, R. ; Rimoldi, D. ; Colombo, M. P. ; Jasmin, C. ; Carrel, S. ; Azzarone, B. / Human melanoma cells express a functional interleukin-2 receptor. In: International Journal of Cancer. 1993 ; Vol. 55, No. 1. pp. 164-170.
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abstract = "Flow cytometric analysis reveals that 5 human melanoma cell lines (M14, IGR3, ME1477, JUSO, GLL19) express both α and β chain of the interleukin 2 receptor (IL-2Rα and IL-2Rβ). These chains are able to specifically bind IL-2 and to form high-affinity heterodimers (IL-2Rαβ). Analysis of poly A+ RNAs by Northern blot reveals the presence of typical transcripts for both the IL-2Rα gene (3.6 kb) and the IL-2Rβ gene (4 kb). Reverse transcription/polymerase chain reaction analysis allowed transcripts for the IL-2Rγ (p64) gene to be detected in 3 of these melanoma cell lines (M14, IGR3, ME1477). Incubation with human recombinant IL-2 modifies in IL-2Rα+β+γ+ (M14) the expression of several surface molecules: down-regulation of ICAM-1, HLA class I and HLA-DR and up-regulation of CD44. IL-2 is also active on IL-2α+β+γ- cell lines since it decreases ICAM-1 and HLA class-II expression at the surface of JUSO cells. Down-regulation of ICAM-1, whose expression in melanoma cells is a marker of tumor progression, is detectable within 3 hr in M14 cells and is maximal after 48 hr incubation, at IL-2 concentrations corresponding to the high-affinity heterodimers. This feature is specific since it is partially inhibited by MAbs directed against the IL-2 binding site of the IL-2Rα (MAR93, I0T14) and IL-2Rβ (MiKβ1, TU27) chains. Our data support the notion of a direct effect of IL-2 on human melanoma cells. Modulation of the expression of surface molecules which is important for the interaction with immunocompetent cells or for tumor progression, could have a role to play during in vivo IL-2 treatment of human melanomas.",
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AU - Plaisance, S.

AU - Rubinstein, E.

AU - Alileche, A.

AU - Han, D. S.

AU - Sahraoui, Y.

AU - Mingari, M. C.

AU - Bellomo, R.

AU - Rimoldi, D.

AU - Colombo, M. P.

AU - Jasmin, C.

AU - Carrel, S.

AU - Azzarone, B.

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N2 - Flow cytometric analysis reveals that 5 human melanoma cell lines (M14, IGR3, ME1477, JUSO, GLL19) express both α and β chain of the interleukin 2 receptor (IL-2Rα and IL-2Rβ). These chains are able to specifically bind IL-2 and to form high-affinity heterodimers (IL-2Rαβ). Analysis of poly A+ RNAs by Northern blot reveals the presence of typical transcripts for both the IL-2Rα gene (3.6 kb) and the IL-2Rβ gene (4 kb). Reverse transcription/polymerase chain reaction analysis allowed transcripts for the IL-2Rγ (p64) gene to be detected in 3 of these melanoma cell lines (M14, IGR3, ME1477). Incubation with human recombinant IL-2 modifies in IL-2Rα+β+γ+ (M14) the expression of several surface molecules: down-regulation of ICAM-1, HLA class I and HLA-DR and up-regulation of CD44. IL-2 is also active on IL-2α+β+γ- cell lines since it decreases ICAM-1 and HLA class-II expression at the surface of JUSO cells. Down-regulation of ICAM-1, whose expression in melanoma cells is a marker of tumor progression, is detectable within 3 hr in M14 cells and is maximal after 48 hr incubation, at IL-2 concentrations corresponding to the high-affinity heterodimers. This feature is specific since it is partially inhibited by MAbs directed against the IL-2 binding site of the IL-2Rα (MAR93, I0T14) and IL-2Rβ (MiKβ1, TU27) chains. Our data support the notion of a direct effect of IL-2 on human melanoma cells. Modulation of the expression of surface molecules which is important for the interaction with immunocompetent cells or for tumor progression, could have a role to play during in vivo IL-2 treatment of human melanomas.

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