TY - JOUR
T1 - Human microvascular endothelial cells from different fetal organs demonstrate organ-specific CAM expression
AU - Invernici, G.
AU - Ponti, D.
AU - Corsini, E.
AU - Cristini, S.
AU - Frigerio, S.
AU - Colombo, A.
AU - Parati, E.
AU - Alessandri, G.
PY - 2005/8/15
Y1 - 2005/8/15
N2 - In this work, we isolated and produced long-term cultures of human fetal endothelial cells (fECs) deriving from different organs of the same 12-week-old embryos. Highly pure endothelium cultures were obtained from specimens of brain, heart, lung, liver, aorta and kidney by using magnetic microspheres coated with CD31 or CD34 specific endothelial antibodies. The endothelial nature of these cells was confirmed by the presence of von Willebrand Factor (vWf), Flk-1/VEGFR2 and CD31. The fECs cultures showed organ-specific differences as regards to the morphological appearance, the growth rate and the expression of cellular adhesion molecules (CAMs) before or after stimulation by the inflammatory cytokines IL-1β and TNF-α. For instance, TNF-α showed a specific effect on fetal heart ECs by stimulating E-selectin expression. Our findings indicate that fECs may represent an innovative tool to study differences among ECs of different vascular districts of the same individual, thus increasing the possibility to compare many pathological aspects of human adult and fetal microvasculature.
AB - In this work, we isolated and produced long-term cultures of human fetal endothelial cells (fECs) deriving from different organs of the same 12-week-old embryos. Highly pure endothelium cultures were obtained from specimens of brain, heart, lung, liver, aorta and kidney by using magnetic microspheres coated with CD31 or CD34 specific endothelial antibodies. The endothelial nature of these cells was confirmed by the presence of von Willebrand Factor (vWf), Flk-1/VEGFR2 and CD31. The fECs cultures showed organ-specific differences as regards to the morphological appearance, the growth rate and the expression of cellular adhesion molecules (CAMs) before or after stimulation by the inflammatory cytokines IL-1β and TNF-α. For instance, TNF-α showed a specific effect on fetal heart ECs by stimulating E-selectin expression. Our findings indicate that fECs may represent an innovative tool to study differences among ECs of different vascular districts of the same individual, thus increasing the possibility to compare many pathological aspects of human adult and fetal microvasculature.
KW - Cell adhesion molecules
KW - Comparative studies
KW - Endothelium
KW - Heterogeneity
KW - In vitro
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U2 - 10.1016/j.yexcr.2005.04.033
DO - 10.1016/j.yexcr.2005.04.033
M3 - Article
C2 - 15936757
AN - SCOPUS:24944510515
VL - 308
SP - 273
EP - 282
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -