Human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable EBV-transformed cell clone

A. Amadori, V. Criminale, M. L. Calabro, L. Tessarollo, E. Francavilla, L. Chieco-Bianchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An EBV-transformed lymphoblastoid B cell clone (A12) derived from peripheral blood lymphocytes of an HIV-1-infected individual is described. The immunoglobulin isotype produced by this clone was IgM, and Southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. The A12 monoclonal antibody was specific for the p24 product of the HIV-1 gag gene. This clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume5
Issue number1
Publication statusPublished - 1989

Fingerprint

Human Immunodeficiency Virus gag Gene Products
Human Herpesvirus 4
compound A 12
Clone Cells
Monoclonal Antibodies
HIV-1
HIV Core Protein p24
gag Genes
Immunoglobulin Genes
Gene Rearrangement
Immunoglobulin Isotypes
Southern Blotting
Immunoglobulin M
B-Lymphocytes
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable EBV-transformed cell clone. / Amadori, A.; Criminale, V.; Calabro, M. L.; Tessarollo, L.; Francavilla, E.; Chieco-Bianchi, L.

In: AIDS Research and Human Retroviruses, Vol. 5, No. 1, 1989, p. 73-78.

Research output: Contribution to journalArticle

@article{2d6882032b9749149ce4991ddcae9df9,
title = "Human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable EBV-transformed cell clone",
abstract = "An EBV-transformed lymphoblastoid B cell clone (A12) derived from peripheral blood lymphocytes of an HIV-1-infected individual is described. The immunoglobulin isotype produced by this clone was IgM, and Southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. The A12 monoclonal antibody was specific for the p24 product of the HIV-1 gag gene. This clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed.",
author = "A. Amadori and V. Criminale and Calabro, {M. L.} and L. Tessarollo and E. Francavilla and L. Chieco-Bianchi",
year = "1989",
language = "English",
volume = "5",
pages = "73--78",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - Human monoclonal antibody against a gag-coded protein of human immunodeficiency virus produced by a stable EBV-transformed cell clone

AU - Amadori, A.

AU - Criminale, V.

AU - Calabro, M. L.

AU - Tessarollo, L.

AU - Francavilla, E.

AU - Chieco-Bianchi, L.

PY - 1989

Y1 - 1989

N2 - An EBV-transformed lymphoblastoid B cell clone (A12) derived from peripheral blood lymphocytes of an HIV-1-infected individual is described. The immunoglobulin isotype produced by this clone was IgM, and Southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. The A12 monoclonal antibody was specific for the p24 product of the HIV-1 gag gene. This clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed.

AB - An EBV-transformed lymphoblastoid B cell clone (A12) derived from peripheral blood lymphocytes of an HIV-1-infected individual is described. The immunoglobulin isotype produced by this clone was IgM, and Southern blot analysis of immunoglobulin gene rearrangement showed a monoclonal pattern. The A12 monoclonal antibody was specific for the p24 product of the HIV-1 gag gene. This clone is now in continuous culture for more than 8 months and no changes in its biologic properties have been observed.

UR - http://www.scopus.com/inward/record.url?scp=0024521023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024521023&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 73

EP - 78

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 1

ER -