Human monocytoid THP-1 cell line versus monocyte-derived human immature dendritic cells as in vitro models for predicting the sensitising potential of chemicals

E. Bocchietto, C. Paolucci, D. Breda, E. Sabbioni, Samuele E. Burastero

Research output: Contribution to journalArticle

Abstract

Immature dendritic cells (DCs) modulate differentiation markers following in vitro exposure to chemicals generating contact allergies. THP-1 is a monocytoid cell line maintaining some differentiating plasticity. In this study, human DCs and THP-1 cells were compared as in vitro models to predict contact sensitisation of chemicals with different sensitising potential. Expression of CD80 and CD86 was assessed by flow cytometry after exposure to subtoxic concentrations of potent (2,4-dinitrochlorobenzene, DNCB and p-phenylendiamine, PPD), strong (thimerosal, TMS), moderate (sodium tetrachloroplatinate, Na 2PtCl4) sensitising compounds as well as of non-sensitising, irritating sodium dodecyl sulphate (SDS) as compared to a vehicle of sensitising substances (dimethyl sulphoxide, DMSO). Up-regulation of CD86 following in vitro incubation of DCs and THP-1 cells with DNCB, PPD, TMS and Na2PtCl4 but not with SDS, was observed. The CD80 membrane marker was up-regulated on DCs following in vitro incubation with DNCB and PPD, but not with TMS, Na2PtCl4 and SDS. On THP-1 cells, only DNCR up-regulated CD80 expression. In conclusion, both the cell line THP-1 and DCs are promising in vitro models for assays aiming at predicting the sensitisation potential of chemicals. THP-1 cell line is by far easier to handle and offers relevant advantages from the practical point of view.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume20
Issue number2
Publication statusPublished - Apr 2007

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Dinitrochlorobenzene
Dendritic Cells
Monocytes
Thimerosal
Tuberculin
Cell Line
Sodium Dodecyl Sulfate
Differentiation Antigens
Dimethyl Sulfoxide
Cell Differentiation
Flow Cytometry
Hypersensitivity
Up-Regulation
Sodium
In Vitro Techniques
Membranes

Keywords

  • Allergotoxicity
  • Dendritic cell line
  • In vitro sensitisation testing
  • Skin sensitization
  • THP-1 cell line

ASJC Scopus subject areas

  • Pharmacology

Cite this

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title = "Human monocytoid THP-1 cell line versus monocyte-derived human immature dendritic cells as in vitro models for predicting the sensitising potential of chemicals",
abstract = "Immature dendritic cells (DCs) modulate differentiation markers following in vitro exposure to chemicals generating contact allergies. THP-1 is a monocytoid cell line maintaining some differentiating plasticity. In this study, human DCs and THP-1 cells were compared as in vitro models to predict contact sensitisation of chemicals with different sensitising potential. Expression of CD80 and CD86 was assessed by flow cytometry after exposure to subtoxic concentrations of potent (2,4-dinitrochlorobenzene, DNCB and p-phenylendiamine, PPD), strong (thimerosal, TMS), moderate (sodium tetrachloroplatinate, Na 2PtCl4) sensitising compounds as well as of non-sensitising, irritating sodium dodecyl sulphate (SDS) as compared to a vehicle of sensitising substances (dimethyl sulphoxide, DMSO). Up-regulation of CD86 following in vitro incubation of DCs and THP-1 cells with DNCB, PPD, TMS and Na2PtCl4 but not with SDS, was observed. The CD80 membrane marker was up-regulated on DCs following in vitro incubation with DNCB and PPD, but not with TMS, Na2PtCl4 and SDS. On THP-1 cells, only DNCR up-regulated CD80 expression. In conclusion, both the cell line THP-1 and DCs are promising in vitro models for assays aiming at predicting the sensitisation potential of chemicals. THP-1 cell line is by far easier to handle and offers relevant advantages from the practical point of view.",
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AU - Bocchietto, E.

AU - Paolucci, C.

AU - Breda, D.

AU - Sabbioni, E.

AU - Burastero, Samuele E.

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