TY - JOUR
T1 - Human mononuclear phagocytes from different anatomical sites differ in their capacity to metabolize arachidonic acid
AU - Vicenzi, E.
AU - Biondi, A.
AU - Bordignon, C.
AU - Rambaldi, A.
AU - Donati, M. B.
AU - Mantovani, A.
PY - 1984
Y1 - 1984
N2 - Human mononuclear phagocytes have the capacity to metabolize arachidonic acid (AA) into prostaglandin (PG) endowed with potent activities in immune responses and inflammatory processes. We have evaluated AA metabolism in human mononuclear phagocytes harvested from different anatomical sites (blood monocytes, macrophages from milk, peritoneal activity and alveolar spaces). At thin layer radiochromatography, the AA metabolites mainly present were PGE2, TxB2 and, only in bronchoalveolar macrophages, a peak comigrating with PGD2. No appreciable levels of 6-keto-PGF (1α) were observed. These data were confirmed using specific radioimmunoassays for TxB2, PGE2, and 6-keto-PGF(1α). Blood monocytes and bronchoalveolar macrophages were the poorest producers of PG, with the possible exception of PGD2 in bronchoalveolar macrophages. The high amounts of TxB2 and PGE2 produced by milk macrophages could contribute to the levels of PG in human milk. Thus, human mononuclear phagocytes obtained from diverse anatomical sites are considerably heterogeneous in terms of AA metabolism.
AB - Human mononuclear phagocytes have the capacity to metabolize arachidonic acid (AA) into prostaglandin (PG) endowed with potent activities in immune responses and inflammatory processes. We have evaluated AA metabolism in human mononuclear phagocytes harvested from different anatomical sites (blood monocytes, macrophages from milk, peritoneal activity and alveolar spaces). At thin layer radiochromatography, the AA metabolites mainly present were PGE2, TxB2 and, only in bronchoalveolar macrophages, a peak comigrating with PGD2. No appreciable levels of 6-keto-PGF (1α) were observed. These data were confirmed using specific radioimmunoassays for TxB2, PGE2, and 6-keto-PGF(1α). Blood monocytes and bronchoalveolar macrophages were the poorest producers of PG, with the possible exception of PGD2 in bronchoalveolar macrophages. The high amounts of TxB2 and PGE2 produced by milk macrophages could contribute to the levels of PG in human milk. Thus, human mononuclear phagocytes obtained from diverse anatomical sites are considerably heterogeneous in terms of AA metabolism.
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M3 - Article
C2 - 6432383
AN - SCOPUS:0021174121
VL - 57
SP - 385
EP - 392
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
SN - 0009-9104
IS - 2
ER -