Human mononuclear phagocytes from different anatomical sites differ in their capacity to metabolize arachidonic acid

E. Vicenzi, A. Biondi, C. Bordignon, A. Rambaldi, M. B. Donati, A. Mantovani

Research output: Contribution to journalArticlepeer-review

Abstract

Human mononuclear phagocytes have the capacity to metabolize arachidonic acid (AA) into prostaglandin (PG) endowed with potent activities in immune responses and inflammatory processes. We have evaluated AA metabolism in human mononuclear phagocytes harvested from different anatomical sites (blood monocytes, macrophages from milk, peritoneal activity and alveolar spaces). At thin layer radiochromatography, the AA metabolites mainly present were PGE2, TxB2 and, only in bronchoalveolar macrophages, a peak comigrating with PGD2. No appreciable levels of 6-keto-PGF (1α) were observed. These data were confirmed using specific radioimmunoassays for TxB2, PGE2, and 6-keto-PGF(1α). Blood monocytes and bronchoalveolar macrophages were the poorest producers of PG, with the possible exception of PGD2 in bronchoalveolar macrophages. The high amounts of TxB2 and PGE2 produced by milk macrophages could contribute to the levels of PG in human milk. Thus, human mononuclear phagocytes obtained from diverse anatomical sites are considerably heterogeneous in terms of AA metabolism.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalClinical and Experimental Immunology
Volume57
Issue number2
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Immunology

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