Human neoplastic mesothelial cells express voltage-gated sodium channels involved in cell motility

Gianluca Fulgenzi, Laura Graciotti, Monica Faronato, Maria Virginia Soldovieri, Francesco Miceli, Salvatore Amoroso, Lucio Annunziato, Antonio Procopio, Maurizio Taglialatela

Research output: Contribution to journalArticlepeer-review

Abstract

Given the pivotal role of ion channels in neoplastic transformation, the aim of the present study has been to assess possible differences in the expression patterns of voltage-gated monovalent cationic (Na+ and K+) currents between normal and neoplastic mesothelial cells (NM, MPM, respectively), and to evaluate the role of specific ion channels in mesothelioma cells proliferation, apoptosis, and motility. To achieve this aim, membrane currents expressed in NM and MPM cells derived from surgically-removed human specimens were investigated by means of patch-clamp electrophysiology. NM cells were found to express three main classes of K+ currents, which were defined as KIR, maxiKCa, and KV currents on the basis of their biophysical and pharmacological properties. Each of these K+ currents was absent in MPM cells; by contrast, MPM cells revealed the novel appearance of tetrodotoxin (TTX)-sensitive voltage-gated Na+ currents undetected in normal mesothelial cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR analysis of MPM cells transcripts showed significant expression of the mRNAs encoding for NaV1.2, and NaV1.6, and NaV1.7 (and less so for NaV1.3, NaV1.4, and NaV1.5) main voltage-gated sodium channel (VGSC) α-subunit(s). Interestingly, blockade of VGSCs with TTX decreased mesothelioma cell migration in in vitro motility assays; on the other hand, TTX failed to interfere with cell viability, proliferation, and apoptosis progression triggered by UV exposure. In summary, the results of the present study suggest that VGSCs expression in MPM cells may favor the increased motility of the neoplastic cells, a phenotypic feature often associated with the malignant phenotype.

Original languageEnglish
Pages (from-to)1146-1159
Number of pages14
JournalInternational Journal of Biochemistry and Cell Biology
Volume38
Issue number7
DOIs
Publication statusPublished - 2006

Keywords

  • Mesothelioma
  • Mesothelium
  • Patch-clamp
  • Voltage-gated potassium channel
  • Voltage-gated sodium channel

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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