TY - JOUR
T1 - Human neoplastic mesothelial cells express voltage-gated sodium channels involved in cell motility
AU - Fulgenzi, Gianluca
AU - Graciotti, Laura
AU - Faronato, Monica
AU - Soldovieri, Maria Virginia
AU - Miceli, Francesco
AU - Amoroso, Salvatore
AU - Annunziato, Lucio
AU - Procopio, Antonio
AU - Taglialatela, Maurizio
PY - 2006
Y1 - 2006
N2 - Given the pivotal role of ion channels in neoplastic transformation, the aim of the present study has been to assess possible differences in the expression patterns of voltage-gated monovalent cationic (Na+ and K+) currents between normal and neoplastic mesothelial cells (NM, MPM, respectively), and to evaluate the role of specific ion channels in mesothelioma cells proliferation, apoptosis, and motility. To achieve this aim, membrane currents expressed in NM and MPM cells derived from surgically-removed human specimens were investigated by means of patch-clamp electrophysiology. NM cells were found to express three main classes of K+ currents, which were defined as KIR, maxiKCa, and KV currents on the basis of their biophysical and pharmacological properties. Each of these K+ currents was absent in MPM cells; by contrast, MPM cells revealed the novel appearance of tetrodotoxin (TTX)-sensitive voltage-gated Na+ currents undetected in normal mesothelial cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR analysis of MPM cells transcripts showed significant expression of the mRNAs encoding for NaV1.2, and NaV1.6, and NaV1.7 (and less so for NaV1.3, NaV1.4, and NaV1.5) main voltage-gated sodium channel (VGSC) α-subunit(s). Interestingly, blockade of VGSCs with TTX decreased mesothelioma cell migration in in vitro motility assays; on the other hand, TTX failed to interfere with cell viability, proliferation, and apoptosis progression triggered by UV exposure. In summary, the results of the present study suggest that VGSCs expression in MPM cells may favor the increased motility of the neoplastic cells, a phenotypic feature often associated with the malignant phenotype.
AB - Given the pivotal role of ion channels in neoplastic transformation, the aim of the present study has been to assess possible differences in the expression patterns of voltage-gated monovalent cationic (Na+ and K+) currents between normal and neoplastic mesothelial cells (NM, MPM, respectively), and to evaluate the role of specific ion channels in mesothelioma cells proliferation, apoptosis, and motility. To achieve this aim, membrane currents expressed in NM and MPM cells derived from surgically-removed human specimens were investigated by means of patch-clamp electrophysiology. NM cells were found to express three main classes of K+ currents, which were defined as KIR, maxiKCa, and KV currents on the basis of their biophysical and pharmacological properties. Each of these K+ currents was absent in MPM cells; by contrast, MPM cells revealed the novel appearance of tetrodotoxin (TTX)-sensitive voltage-gated Na+ currents undetected in normal mesothelial cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR analysis of MPM cells transcripts showed significant expression of the mRNAs encoding for NaV1.2, and NaV1.6, and NaV1.7 (and less so for NaV1.3, NaV1.4, and NaV1.5) main voltage-gated sodium channel (VGSC) α-subunit(s). Interestingly, blockade of VGSCs with TTX decreased mesothelioma cell migration in in vitro motility assays; on the other hand, TTX failed to interfere with cell viability, proliferation, and apoptosis progression triggered by UV exposure. In summary, the results of the present study suggest that VGSCs expression in MPM cells may favor the increased motility of the neoplastic cells, a phenotypic feature often associated with the malignant phenotype.
KW - Mesothelioma
KW - Mesothelium
KW - Patch-clamp
KW - Voltage-gated potassium channel
KW - Voltage-gated sodium channel
UR - http://www.scopus.com/inward/record.url?scp=33645848140&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645848140&partnerID=8YFLogxK
U2 - 10.1016/j.biocel.2005.12.003
DO - 10.1016/j.biocel.2005.12.003
M3 - Article
C2 - 16458569
AN - SCOPUS:33645848140
VL - 38
SP - 1146
EP - 1159
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
SN - 1357-2725
IS - 7
ER -