Human neuronal cell viability demonstrated in culture after cryopreservation

Vincenzo Silani, Antonio Pizzuti, Ornella Strada, Andrea Falini, Mauro Buscaglia, Guglielmo Scarlato

Research output: Contribution to journalArticlepeer-review


A human neuronal cell freezing technique has been developed. The results indicate that human fetal neuronal cells can be frozen with 7%-10% dimethyl sulfoxide (DMSO) as cryoprotectant. The survival of isolated thawed cells has been evaluated in culture. Cells have been frozen at -196 °C for 370 days without loss of viability. Average recovery rate of frozen cells was 62% of the recovery rate of cultured unfrozen controls. Thawed cells show neurite outgrowth and maintain both cellular markers such as neurons specific enolase (NSE) and neurochemical characteristics (GABA synthesis). Morphological integrity of cryopreserved neurons has been confirmed at ultrastructural level.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalBrain Research
Issue number1
Publication statusPublished - Nov 8 1988


  • Cryopreservation
  • Neuron-specific enolase
  • Neuronal cell
  • Tissue culture
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)


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