Human orbital tissue and thyroid membranes express a 64 kDa protein which is recognized by autoantibodies in the serum of patients with thyroid-associated ophthalmopathy

M. Salvi, A. Miller, J. R. Wall

Research output: Contribution to journalArticle

Abstract

A 64 kDa protein has been identified in the membrane fraction of human eye muscle, orbital connective tissue and thyroid, by testing sera of patients with thyroid-associated ophthalmopathy in SDS-polyacrylamide gel electrophoresis and Western blotting. Antibodies to this membrane antigen seem characteristic of the early stage of ophthalmopathy. In the thyroid this newly recognized protein seems different from previously known membrane antigens. A thyroid antibody reactive with a 64 kDa membrane antigen in eye muscle could explain the very frequent association of ophthalmopathy with autoimmune thyroid disease.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalFEBS Letters
Volume232
Issue number1
DOIs
Publication statusPublished - May 9 1988

Fingerprint

Graves Ophthalmopathy
Autoantibodies
Thyroid Gland
Tissue
Membranes
Serum
Antigens
Muscle
Proteins
Muscles
Antibodies
Thyroid Diseases
Electrophoresis
Connective Tissue
Autoimmune Diseases
Polyacrylamide Gel Electrophoresis
Western Blotting
Association reactions
Testing

Keywords

  • 64 kDa protein
  • Autoantibody
  • Graves' disease
  • Ophthalmopathy
  • Orbital antigen

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AU - Wall, J. R.

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AB - A 64 kDa protein has been identified in the membrane fraction of human eye muscle, orbital connective tissue and thyroid, by testing sera of patients with thyroid-associated ophthalmopathy in SDS-polyacrylamide gel electrophoresis and Western blotting. Antibodies to this membrane antigen seem characteristic of the early stage of ophthalmopathy. In the thyroid this newly recognized protein seems different from previously known membrane antigens. A thyroid antibody reactive with a 64 kDa membrane antigen in eye muscle could explain the very frequent association of ophthalmopathy with autoimmune thyroid disease.

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KW - Ophthalmopathy

KW - Orbital antigen

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