Human osteoblast cultures from osteoporotic and healthy bone: Biochemical markers and cytokine expression in basal conditions and in response to 1,25(OH)2D3

Research output: Contribution to journalArticle

Abstract

The development of methods to culture bone cells has enhanced in vitro studies and allowed researchers to investigate bone cell metabolism in healthy tissue and in various different bone diseases. Greater knowledge of cultures of pathologic bone tissue-derived osteoblasts may be helpful in performing in vitro experiments that test biomaterials and therapies to be used in the orthopedic field, since this kind of approach better reflects the conditions of clinical relevance to many patients. In the present study primary cultures of human osteoblastic cells were isolated from donors with osteoporosis (HOB, Human Osteopenic Bone) and their respective controls (HNB, Human Normal Bone). They were then characterized in baseline conditions and after stimulation with 10-9 M 1,25(OH2)D3. Specific biochemical markers of bone cells and cytokines involved in bone turnover were evaluated to assess cell metabolism and any possible differences between osteoblasts derived from healthy and osteopenic bone tissue. Under baseline conditions, HNB and HOB in vitro cultures showed some differences in proliferation (MTT test), PICP, OC and IL-6. The HNB response to 1,25(OH2)D3 stimulation differed significantly from that of the HOB cultures but only with regard to the MTT test, and ALP and PICP levels; the other selected parameters showed a similar behavior for both cultures. The current findings should be taken into account when cultures derived from human bone are used for in vitro experiments.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume30
Issue number3
DOIs
Publication statusPublished - 2002

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Osteoblasts
Cell culture
Bone
Biomarkers
Cytokines
Bone and Bones
Tissue
Metabolism
Bone Remodeling
Bone Diseases
Biocompatible Materials
Orthopedics
Osteoporosis
Interleukin-6
Biomaterials
Cell Culture Techniques
Research Personnel
Tissue Donors

ASJC Scopus subject areas

  • Biomedical Engineering
  • Hematology
  • Biotechnology
  • Biomaterials

Cite this

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abstract = "The development of methods to culture bone cells has enhanced in vitro studies and allowed researchers to investigate bone cell metabolism in healthy tissue and in various different bone diseases. Greater knowledge of cultures of pathologic bone tissue-derived osteoblasts may be helpful in performing in vitro experiments that test biomaterials and therapies to be used in the orthopedic field, since this kind of approach better reflects the conditions of clinical relevance to many patients. In the present study primary cultures of human osteoblastic cells were isolated from donors with osteoporosis (HOB, Human Osteopenic Bone) and their respective controls (HNB, Human Normal Bone). They were then characterized in baseline conditions and after stimulation with 10-9 M 1,25(OH2)D3. Specific biochemical markers of bone cells and cytokines involved in bone turnover were evaluated to assess cell metabolism and any possible differences between osteoblasts derived from healthy and osteopenic bone tissue. Under baseline conditions, HNB and HOB in vitro cultures showed some differences in proliferation (MTT test), PICP, OC and IL-6. The HNB response to 1,25(OH2)D3 stimulation differed significantly from that of the HOB cultures but only with regard to the MTT test, and ALP and PICP levels; the other selected parameters showed a similar behavior for both cultures. The current findings should be taken into account when cultures derived from human bone are used for in vitro experiments.",
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AU - Giardino, Roberto

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