Human osteoblast response to pulsed laser deposited calcium phosphate coatings

A. Bigi, B. Bracci, F. Cuisinier, R. Elkaim, M. Fini, I. Mayer, I. N. Mihailescu, G. Socol, L. Sturba, P. Torricelli

Research output: Contribution to journalArticlepeer-review


Octacalcium phosphate (OCP) and Mn 2+-doped carbonate hydroxyapatite (Mn-CHA) thin films were deposited on pure, highly polished and chemically etched Ti substrates with pulsed laser deposition. The coatings exhibit different composition, crystallinity and morphology that might affect their osteoconductivity. Human osteoblasts were cultured on the surfaces of OCP and Mn-CHA thin films, and the cell attachment, proliferation and differentiation were evaluated up to 21 days. The cells showed a normal morphology and a very good rate of proliferation and viability in every experimental time. Alkaline phosphatase activity was always higher than the control and Ti groups. From days 7 to 21 collagen type I production was higher in comparison with control and Ti groups. The level of transforming growth factor beta 1 (TGF-β1) was lower at 3 and 7 days, but reached the highest values during following experimental times (14 and 21 days). Our data demonstrate that both calcium phosphate coatings favour osteoblasts proliferation, activation of their metabolism and differentiation.

Original languageEnglish
Pages (from-to)2381-2389
Number of pages9
Issue number15
Publication statusPublished - May 2005


  • Calcium phosphate coating
  • Differentiation
  • Human osteoblasts
  • Laser ablation
  • Mn -doped carbonated hydroxyapatite
  • Octacalcium phosphate

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering


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