Human papillomavirus type 16 E6-enhanced susceptibility to apoptosis induced by TNF in A2780 human ovarian cancer cell line

Faina Vikhanskaya, Carla Falugi, Piera Valente, Patrizia Russo

Research output: Contribution to journalArticle

Abstract

In our study, we show that expression of HPV-16 E6 sensitizes TNF-induced cytotoxicity of human ovarian cancer cell line A2780. This effect is not related to a different number of TNF receptors present on cell membrane. The major induction of massive apoptosis induced by TNF is not p53-and p21Waf-1-dependent but it is principally related to NF-κB inhibition in A2780/E6 cells. Consistently to NF-KB inhibition a rapidly release of cytochrome c and severe induction of DNA fragmentation are seen in A2780/E6 cells. Also in human colon cancer cell line HCT-116/E6 the expression of HPV-16 E6 enhances TNF-cytotoxicity. This effect is not present in the HCT-116/mu-p53 clone (transfected with a dominant-negative mutated p53 transgene). Thus, taken together all these observations suggest that HPV-16 E6 sensitizes A2780 and HCT-116 cells to TNF; this effect is not p53-dependent, but it is essentially mediated through an inhibition in activating NF-κB activities.

Original languageEnglish
Pages (from-to)732-739
Number of pages8
JournalInternational Journal of Cancer
Volume97
Issue number6
DOIs
Publication statusPublished - Feb 20 2002

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Keywords

  • Apoptosis
  • Cytochrome c
  • HPV-16 E6
  • NF-κB
  • TNF

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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