TY - JOUR
T1 - Human papillomavirus type 32 does not display in Vitro transforming properties
AU - Caldeira, Sandra
AU - Dong, Wen
AU - Tomakidi, Pascal
AU - Paradiso, Angelo
AU - Tommasino, Massimo
PY - 2002
Y1 - 2002
N2 - Human papillomavirus type 32 (HPV32) is one of the etiological agents of a benign oral condition, focal epithelial hyperplasia. However, the previously characterized properties of its E7 oncoprotein suggest a possible malignant nature for this virus. In this study we characterized the properties of HPV32 E6 and E7. Our data show that HPV32 E7, despite its high affinity for pRb, does not promote degradation of the cellular protein. In addition, HPV32 E6 does not prevent p53-mediated apoptosis and/or cell cycle arrest. Moreover, coexpression of HPV32 E6 and E7 in primary human fibroblasts or keratinocytes does not alter their proliferative state. Together, these data provide evidence of the benign nature of HPV32.
AB - Human papillomavirus type 32 (HPV32) is one of the etiological agents of a benign oral condition, focal epithelial hyperplasia. However, the previously characterized properties of its E7 oncoprotein suggest a possible malignant nature for this virus. In this study we characterized the properties of HPV32 E6 and E7. Our data show that HPV32 E7, despite its high affinity for pRb, does not promote degradation of the cellular protein. In addition, HPV32 E6 does not prevent p53-mediated apoptosis and/or cell cycle arrest. Moreover, coexpression of HPV32 E6 and E7 in primary human fibroblasts or keratinocytes does not alter their proliferative state. Together, these data provide evidence of the benign nature of HPV32.
KW - Cell cycle and apoptosis
KW - E6 and E7
KW - Focal epithelial hyperplasia
KW - HPV32
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U2 - 10.1006/viro.2002.1623
DO - 10.1006/viro.2002.1623
M3 - Article
C2 - 12359456
AN - SCOPUS:0036394920
VL - 301
SP - 157
EP - 164
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -