Human platelet aggregation by Thrombofax: an electron microscopic study of the sequence of events

F. De Clerck, M. Borgers, J. Vermylen, G. De Gaetano

Research output: Contribution to journalArticlepeer-review

Abstract

The ultrastructural changes and the kinetics of 14C serotonin release during the adhesion aggregation reaction of human platelets induced by Thrombofax (Ortho Pharmaceuticals), a commercial cephalin prepared as an ether extract of acetone dried bovine brain, were analyzed. The initial phase of interaction of Thrombofax with platelets is characterized by the development of adhesion sites on normally shaped cells; thereafter, loss of discoid shape, pseudopod formation and inward movement of granules, surrounded by a circumferential bundle of microtubules, become apparent. Measurements of 14C serotonin release show that these ultrastructural changes precede the release reaction. The later stages which are detectable turbidometrically and which only occur in stirred samples and in the absence of release inhibitors, are characterized mainly by large aggregate formation, platelet degranulation and strong release of 14C serotonin.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalScandinavian Journal of Haematology
Volume12
Issue number2
Publication statusPublished - 1974

ASJC Scopus subject areas

  • Hematology

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