Human platelets express authentic CB1 and CB2 receptors

M. V. Catani, V. Gasperi, G. Catanzaro, S. Baldassarri, A. Bertoni, F. Sinigaglia, L. Avigliano, M. MacCarrone

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In the last decade, the neurovascular effects exerted by endocannabinoids (eCBs) have attracted growing interest, because they hold the promise to open new avenues of therapeutic intervention against major causes of death in Western society. Several actions of eCBs are mediated by type-1 (CB1) or type-2 (CB2) cannabinoid receptors, yet there is no clear evidence of the presence of these proteins in platelets. To demonstrate that CB1 and CB2 are expressed in human platelets, we analyzed their protein level by Western blotting and ELISA, visualized their cellular localization by confocal microscopy, and ascertained their functionality by binding assays. We found that CB1, and to a lesser extent CB2, are expressed in highly purified human platelets. Both receptor subtypes were predominantly localized inside the cell, thus explaining why they might remain undetected in preparations of plasma membranes. The identification of authentic CB1 and CB2 in human platelets supports the potential exploitation of selective agonists or antagonists of these receptors as novel therapeutics to combat neurovascular disorders. It seems remarkable that some of these substances have been already used in humans to treat disease states.

Original languageEnglish
Pages (from-to)311-318
Number of pages8
JournalCurrent Neurovascular Research
Volume7
Issue number4
Publication statusPublished - Nov 2010

Fingerprint

Cannabinoid Receptor CB2
Cannabinoid Receptor CB1
Blood Platelets
Endocannabinoids
Combat Disorders
Confocal Microscopy
Cause of Death
Proteins
Western Blotting
Enzyme-Linked Immunosorbent Assay
Cell Membrane
Therapeutics

Keywords

  • 2-arachidonoylglycerol
  • Anandamide
  • Cannabinoid receptor type-1
  • Cannabinoid receptor type-2
  • Human platelets
  • Neurovascular disease

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Catani, M. V., Gasperi, V., Catanzaro, G., Baldassarri, S., Bertoni, A., Sinigaglia, F., ... MacCarrone, M. (2010). Human platelets express authentic CB1 and CB2 receptors. Current Neurovascular Research, 7(4), 311-318.

Human platelets express authentic CB1 and CB2 receptors. / Catani, M. V.; Gasperi, V.; Catanzaro, G.; Baldassarri, S.; Bertoni, A.; Sinigaglia, F.; Avigliano, L.; MacCarrone, M.

In: Current Neurovascular Research, Vol. 7, No. 4, 11.2010, p. 311-318.

Research output: Contribution to journalArticle

Catani, MV, Gasperi, V, Catanzaro, G, Baldassarri, S, Bertoni, A, Sinigaglia, F, Avigliano, L & MacCarrone, M 2010, 'Human platelets express authentic CB1 and CB2 receptors', Current Neurovascular Research, vol. 7, no. 4, pp. 311-318.
Catani MV, Gasperi V, Catanzaro G, Baldassarri S, Bertoni A, Sinigaglia F et al. Human platelets express authentic CB1 and CB2 receptors. Current Neurovascular Research. 2010 Nov;7(4):311-318.
Catani, M. V. ; Gasperi, V. ; Catanzaro, G. ; Baldassarri, S. ; Bertoni, A. ; Sinigaglia, F. ; Avigliano, L. ; MacCarrone, M. / Human platelets express authentic CB1 and CB2 receptors. In: Current Neurovascular Research. 2010 ; Vol. 7, No. 4. pp. 311-318.
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