Human polyomavirus 6 DNA in the cerebrospinal fluid of an HIV-positive patient with leukoencephalopathy

Serena Delbue, Francesca Elia, Lucia Signorini, Ramona Bella, Sonia Villani, Enrico Marchioni, Pasquale Ferrante, Tung Gia Phan, Eric Delwart

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Leukoencephalopathies in HAART-treated, HIV-positive patients include progressive multifocal leukoencephalopathy (PML), a result of lytic infection oligodendrocytes by JC polyomavirus (JCV), and another form characterized by the absence of JCV genome in cerebrospinal fluid (CSF). Objectives: To test the potential viral etiology of JCV-negative leukoencephalopathy. Study design: CSF was collected from 43 HIV-positive patients with MRI suggestive of leukoencephalopathies. DNA was isolated and real-time PCR assays for neurotropic viruses (Herpes Simplex Viruses 1/2, Varicella Zoster Virus, Epstein Barr Virus, Human Cytomegalovirus, Human Herpesvirus 6, JCV and HIV) were conducted. CSF from 14 non-reactive cases were subjected to random nucleic acid amplification, deep sequencing, and in silico search for viral sequences. Results: JCV genome was detected in the CSF of 19/43 PML patients, HIV genome in the CSF of 5 PML patients including 2 JCV negative patients, and no viruses were detected in 22 patients. Human Polyomavirus 6 (HPyV6) DNA was detected by deep sequencing in one JCV-negative leukoencephalopathy CSF sample. Conclusions: HPyV6 DNA was detected in CSF of a case of demyelinating disease. HPyV6 has not been previously reported in CSF or associated with any disease. Demonstrating a causative role will require further studies.

Original languageEnglish
Pages (from-to)24-27
Number of pages4
JournalJournal of Clinical Virology
Volume68
DOIs
Publication statusPublished - Jul 1 2015

Fingerprint

Leukoencephalopathies
Polyomavirus
JC Virus
Cerebrospinal Fluid
HIV
DNA
Progressive Multifocal Leukoencephalopathy
High-Throughput Nucleotide Sequencing
Genome
Viruses
Human Herpesvirus 6
Human Herpesvirus 3
Human Herpesvirus 2
Oligodendroglia
Highly Active Antiretroviral Therapy
Human Herpesvirus 1
Demyelinating Diseases
Cytomegalovirus
Human Herpesvirus 4
Computer Simulation

Keywords

  • Cerebrospinal fluid
  • Deep sequencing
  • Leukoencephalopathy
  • Polyomaviruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Human polyomavirus 6 DNA in the cerebrospinal fluid of an HIV-positive patient with leukoencephalopathy. / Delbue, Serena; Elia, Francesca; Signorini, Lucia; Bella, Ramona; Villani, Sonia; Marchioni, Enrico; Ferrante, Pasquale; Phan, Tung Gia; Delwart, Eric.

In: Journal of Clinical Virology, Vol. 68, 01.07.2015, p. 24-27.

Research output: Contribution to journalArticle

Delbue, Serena ; Elia, Francesca ; Signorini, Lucia ; Bella, Ramona ; Villani, Sonia ; Marchioni, Enrico ; Ferrante, Pasquale ; Phan, Tung Gia ; Delwart, Eric. / Human polyomavirus 6 DNA in the cerebrospinal fluid of an HIV-positive patient with leukoencephalopathy. In: Journal of Clinical Virology. 2015 ; Vol. 68. pp. 24-27.
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