Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: A pivotal synergy leading to adult bone tissue formation

R. d'Aquino, A. Graziano, M. Sampaolesi, G. Laino, G. Pirozzi, A. De Rosa, G. Papaccio

Research output: Contribution to journalArticle

Abstract

Stromal stem cells from human dental pulp (SBP-DPSCs) were used to study osteogenic differentiation in vitro and in vivo. We previously reported that SBP-DPSCs are multipotent stem cells able to differentiate into osteoblasts, which synthesize three-dimensional woven bone tissue chips in vitro. In this study, we followed the temporal expression pattern of specific markers in SBP-DPSCs and found that, when differentiating into osteoblasts, they express, besides osteocalcin, also flk-1 (VEGF-R2). In addition, 30% of them expressed specific antigens for endothelial cells, including CD54, von-Willebrand (domain 1 and 2), CD31 (PECAM-1) and angiotensin-converting enzyme. Interestingly, we found endotheliocytes forming vessel walls, observing that stem cells synergically differentiate into osteoblasts and endotheliocytes, and that flk-1 exerts a pivotal role in coupling osteoblast and endotheliocyte differentiation. When either SBP-DPSCs or bone chips obtained in vitro were transplanted into immunocompromised rats, they generated a tissue structure with an integral blood supply similar to that of human adult bone; in fact, a large number of HLA-1+ vessels were observed either within the bone or surrounding it in a periosteal layer. This study provides direct evidence to suggest that osteogenesis and angiogenesis mediated by human SBP-DPSCs may be regulated by distinct mechanisms, leading to the organization of adult bone tissue after stem cell transplantion.

Original languageEnglish
Pages (from-to)1162-1171
Number of pages10
JournalCell Death and Differentiation
Volume14
Issue number6
DOIs
Publication statusPublished - Jun 2007

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Dental Pulp
Osteoblasts
Osteogenesis
Bone and Bones
Stem Cells
CD31 Antigens
Multipotent Stem Cells
Osteocalcin
Peptidyl-Dipeptidase A
Stromal Cells
Vascular Endothelial Growth Factor A
Endothelial Cells
Antigens
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

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Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes : A pivotal synergy leading to adult bone tissue formation. / d'Aquino, R.; Graziano, A.; Sampaolesi, M.; Laino, G.; Pirozzi, G.; De Rosa, A.; Papaccio, G.

In: Cell Death and Differentiation, Vol. 14, No. 6, 06.2007, p. 1162-1171.

Research output: Contribution to journalArticle

d'Aquino, R. ; Graziano, A. ; Sampaolesi, M. ; Laino, G. ; Pirozzi, G. ; De Rosa, A. ; Papaccio, G. / Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes : A pivotal synergy leading to adult bone tissue formation. In: Cell Death and Differentiation. 2007 ; Vol. 14, No. 6. pp. 1162-1171.
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